高压脉冲电场提取猪皮胶原蛋白

Extracting Collagen from Pigskin by PEF

为了研究高压脉冲电场(PEF)对提取猪皮胶原蛋白的影响,在料液比1∶10、枯草杆菌中性蛋白酶用量400U/g、酶解温度40℃、pH值6.5的条件下,分别改变电场强度、脉冲频率和酶解时间,用考马斯亮兰法测定胶原蛋白的浓度并计算其溶出率。结果表明,最适电场强度为25kV.cm-1、脉冲频率为40Hz、脉冲时间1s、酶解时间为30min,此条件下胶原蛋白溶出率可达81.3%。经高压脉冲电场处理的猪皮,其胶原蛋白溶出率远高于未经高压脉冲电场处理的的猪皮,而且酶解所用的时间比较短。

The effect of PEF on the yield of extracting collagen from pigskin was studied.The experimental processes were that preprocessed pigskin was treated by PEF at first,hydrolyzed by bacillus subtilis neutral proteinase followed,and the dissolution ratio of collagen was tested by Bradford Method at last.The results showed that the dissolution ratio of the extracting method preprocessed by PEF could be more higher than that of un-preprocessed one,and the preprocessed method could save more enzymolysis time.The dissolution ratio could reach to 81.3% under optimum conditions of material to liquid ratio of 1∶10,enzymes usage of 400U/g,temperature at 40℃,pH value of 6.5,electric field intensity at 25kV·cm^-1,pulsing frequency of 40Hz,pulsing time of 1s and enzymolysis time of 30min.