人巨细胞病毒宫内感染与孕龄的关系

The relationship between human cytomegalovirus infection and gestational age

目的研究孕龄与人巨细胞病毒宫内感染的垂直传播率之间的关系。方法分别应用ELISA方法和PCR方法检测273例不同孕龄的孕妇外周血标本中的HCMV-IgM和HCMV-DNA,新生儿血样或胚胎组织用PCR方法检测HCMV-DNA以诊断先天性HCMV感染。结果孕早期妇女外周血中的HCMV-IgM和HCMV-DNA的阳性率均低于孕中期和孕晚期妇女,差异有显著性(P<0.005)。孕中期和孕晚期妇女宫内感染的垂直传播率显著高于孕早期妇女,差异有统计学意义(P<0.001)。结论孕中期和孕晚期妇女发生宫内感染的几率和垂直传播率均显著大于孕早期妇女,应加强对孕中期和孕晚期HCMV感染孕妇的监测和治疗。

Objective： To explore the relationship between gestational age and vertical transmission of human cytomegalovirus infection.Methods： HCMV-IgM and HCMV-DNA was detected in 273 peripheral blood samples of pregnant women by using ELISA method and PCR method.PCR method was applied to detect HCMV-DNA in newborn blood samples and fetal tissues to diagnose congenital HCMV infection.Results： The positive rates of HCMV-IgM and HCMV-DNA of blood samples of early trimester pregnant women were much lower than middle and late trimester pregnant women.There was statistical difference（P0.005）between them.The vertical transmission rates of intrauterine infection of middle and late trimester pregnant women were much higher than early trimester pregnant women and the difference was statistical（P0.001）.Conclusions： The rates of HCMV intrauterine infection and vertical transmission of middle and late trimester pregnant women were both higher than early trimester pregnant women.It indicates we should pay more attention to the surveillance and treatment of middle and late trimester pregnant women who were HCMV infected.