脐血电泳中Hb溶液浓度对新生儿α-地贫检出结果影响的研究

The study of Hb concentration affectint on the α-thalassemia in cord blood electrophores

目的探讨Hb溶液浓度对α-地中海贫血检出率的影响。方法以PCR检测结果作为金标准,采用美国HELENA公司spife 3000全自动电泳仪对2009年8月～2010年4月在我院产科出生的1450例新生儿脐血标本进行电泳,电泳时将Hb配制成高低两个不同浓度组,并对Hb带进行扫描定量分析。结果基因检测结果1450例脐血标本中,检出静止型51例(3.52%),标准型80例(5.51%),HbH病3例(0.21%),Hb Bart’s水肿胎儿综合征1例(0.061%)。Hb浓度130g/L时,静止型52例(3.59%),标准型82例(5.650%),HbH病3例(0.21%),Hb Bart’s水肿胎儿综合征1例(0.061%)。Hb浓度55-73 g/L时,检出静止型32例(2.21%),标准型77例(5.31%),HbH病3例(0.21%),Hb Bart’s水肿胎儿综合征1例(0.061%)。结论α-地贫标准型、HbH病、Hb Bart’s水肿胎儿综合征的检出率与Hb浓度差异无关;而静止型的检出率与Hb浓度差异有关,高浓度时,检出率高,低浓度时,检出率低。

Objective： Explore the influence of Hb concentration on the α-thalassemia detection rate.Methods：Based on the PCR test result,detect the 400 cord blood samples what born in our hospital＇s antental OPD between 2009-8 to 2010-4 with HELENA company spife 3000 automatic electrophoresis.Hb electrophoresis will be made into high and low concentrations of two different groups,and scanning with quantitative analysis of Hb.Results：From the PCR in 1450 case,we find static 51 cases（3.52%）,standard 80 cases（5.51%）,HbH disease3 cases（0.21%）,Hb Bart＇s hydrops fetalis 1 cases（0.061%）.When Hb concentration is 130g / L,aslo can find static 52cases（3.59%）,standard 82 cases（5.65%）,HbH disease 3 cases（0.21%）,Hb Bart＇s hydrops fetalis 1 cases（0.061%） when Hb concentration is 55-73 g/L;find static 32 cases（2.21%）,standard 77 cases（5.31%）,HbH disease 3 cases（0.21%）,Hb Bart＇s hydrops fetalis 1 cases（0.061%） Conclusion： Hb concentration has no relation of the type of standard,HbH disease,Hb Bart＇s hydrops fetalis;Hb concentration has relation of the type of static.When the Hb concentration is high,the rate of detection is high;when the Hb concentration is low,the rate of detection is low.