吸毒针具残血中HIV抗体检测方法研究

Detction method on HIV antibody of residual blood in drug syringes

目的了解云南边境地区跨境吸毒人群艾滋病感染情况。方法对90份静脉吸毒针具中残血及与之对应的吸毒人员血清样本,260份出入境人员体检样本和90份孕产妇艾滋病监测哨点样本(每份样本包含血清样本和对应的抽血用注射器中残血样本)进行HIV抗体检测。在回收的吸毒针具中,用1.0ml0.01mol/L磷酸盐缓冲盐溶液将回收吸毒针具中残血溶出,检测HIV抗体。结果在回收的吸毒针具残血样本中,HIV抗体检测结果与血清样本检测结果一致。结论对边境地区跨境吸毒人群进行安全注射的宣传教育,以减少边境地区静脉注射吸毒人群共用注射器吸毒的发生,控制艾滋病在吸毒人群中传播。

Objective To learn about HIV infection among cross-border drug users in the border areas of Yunnan. Methods A total of 90 drug syringes and corresponding serum samples of drug addicts, 260 physical examintation samples of personnel going abroad and 90 HIV sentinel surveillance samples of maternal （Each sample contains serum samples and corresponding blood samples of a injector） for HIV antibody test were used. In the recycling of syringes, residual blood was dissolved with 1.0ml 0.01mol/L phosphoric acid buffer salt solution to test HIV antibody. Results In residual blood samples of the drug syringes, HIV antibody test results were consistent with the serum samples. Conclusion It is necessary to carry on the propaganda education of safe injection to cross-border drug users in the border areas, to reduce the incident of sharing syringes to take drugs among intravenous drug users in the border areas, control the spread of AIDS among drug users.