HPLC法测定北刘寄奴中木犀草素和芹菜素的含量

HPLC determination of luteolin and apigenin in Siphonostegia chinensis

目的:建立同时测定北刘寄奴中木犀草素和芹菜素含量的HPLC方法。方法:采用Inertsil ODS-3(4.6 mm×150 mm,5μm)色谱柱,以乙腈-0.5%磷酸(28∶72)为流动相,流速1.0 mL.min-1,检测波长340 nm,柱温35℃。结果:木犀草素和芹菜素进样量分别在0.2168～1.951μg和0.0684～0.615μg范围内与峰面积呈良好的线性关系(r=0.9999);木犀草素和芹菜素平均加样回收率(n=5)分别为99.7%和99.1%,RSD分别为0.99%和1.5%。结论:该方法简便、准确、可靠,可用于中药北刘寄奴中木犀草素和芹菜素含量的测定。

Objective：To develop an HPLC method for content determination of luteolin and apigenin in Siphonostegia chinensis.Methods：The separation was carried out on a Inertsil ODS-3（4.6 mm×150 mm,5 μm） column with acetonitrile-0.5% phosphoric acid（28∶72） as mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was 340 nm and the column temperature was maintained at 35 ℃.Results：There were good linear relationships between the masses and the peak areas of luteolin and apigenin in the ranges of 0.2168-1.951 μg（r=0.9999） and 0.0684-0.615 μg（r=0.9999）,respectively.The average recoveries（n=5） were 99.7% and 99.1%,and RSDs were 0.99% and 1.5%,respectively.Conclusion：This method is simple,precise and reliable for the determination of luteolin and apigenin in Siphonostegia chinensis.