东北鹤虱多糖对免疫抑制小鼠腹腔巨噬细胞功能的影响

Effects of polysaccharide from Lappula Echinata Gilib on peritoneal macrophage in immunosuppressive mice in vitro

目的:研究东北鹤虱多糖(LEGPS-Ⅱ)对免疫抑制小鼠腹腔巨噬细胞(MΦ)功能的影响。方法:采用免疫力低下小鼠,通过中性红实验,观察LEGPS-Ⅱ对小鼠静止和ConA活化的腹腔MΦ体外吞噬功能的影响;采用噻唑蓝比色法(MTT),观察LEG-PS-Ⅱ对小鼠静止和ConA活化的腹腔MΦ体外分泌IL-1β与TNF-α的影响。结果:MΦ体外吞噬实验表明,LEGPS-Ⅱ在0.015 6～0.25μg·mL-1的浓度范围内可剂量依赖性地增加静止和ConA活化的免疫力低下小鼠腹腔MΦ吞噬中性红的能力;LEGPS-Ⅱ在20～80μg·mL-1的浓度范围内可剂量依赖性地增加ConA活化的免疫低下小鼠腹腔MΦ分泌IL-1β,但不能有效刺激免疫低下小鼠静止的腹腔MΦ产生IL-1β;LEGPS-Ⅱ在10～80μg·mL-1的浓度范围内可增加静止和ConA活化的免疫力低下小鼠腹腔MΦ分泌TNF-α,且刺激作用具有剂量依赖性。结论:LEGPS-Ⅱ可以通过提高小鼠腹腔MΦ的吞噬能力,并增加IL-1β与TNF-α的分泌而增强MΦ的免疫调节功能。

Objective： To study the immunoregulatory effects of polysaccharide from Lappula Echinata Gilib （ LEGPS- Ⅱ ） on immunosuppressive murine peritoned macrophage in vitro. Methods： The effects of LEGPS-Ⅱ on phagocytosis of resting and ConA-activated macrophage in immunosuppressive mice were detected with the neutral red method in vitro. The effects of LEGPS- Ⅱ on the eytokine about IL-1β and TNF-α from resting and ConA-activated peritoned macrophage in immunosuppressive mice were assayed by MTr method in vitro. Results： In vitro, the maerophage phagocytosis experiments showed that LEGPS- Ⅱ （0.015 6-0.25μg·mL^-1） had significant enhancement on phagocytosis of resting and ConA-activated peritoned macrophage with the dose-dependent effect. LEGPS-Ⅱ （20- 80 μg·mL^-1） could increase the level of IL-1β secreted by ConA-activated macrophage in immunosuppressive mice, but the level could not be increased in the resting macrophage. LEGPS- Ⅱ（10-80 μg·mL^-1） had obvious enhancement on the TNF-α secretion of resting and ConA-aetivated peritoneal macrophage in immunosuppressive mice with the dose-dependent tendency. Conclusion： These indicate that LEGPS-Ⅱexpresses its specific and nonspecific immunoregulatory effects by improving the phagoeytosis and enhancing the secretion of TNF-α and IL-1β in macrophage in vitro. Key words Lappula Echinata Gilib; Polysaccharide; Immunoregulatory activity; Macrophage; Phagocytosis; Cytokine