长期高脂饲料喂养致小鼠肥胖模型的建立

Establishment of obesity mouse model by long-term high fat diet

目的长期高脂饲料喂养致小鼠肥胖模型并探讨其糖脂代谢的特点。方法采用高脂饲料喂养小鼠三个月,实验结束时测定血糖、血脂、肝体系数和脂体系数,实时荧光定量PCR方法检测肝脏、骨骼肌中脂代谢关键基因mRNA水平。结果长期高脂饲料喂养可升高小鼠的血糖、血脂和脂体系数;降低肝脏、骨骼肌中CPT1 mRNA的表达(P<0.05)。结论长期高脂饲料喂养可引起小鼠的脂代谢紊乱及糖耐量的异常。

Objective To establish the obesity mouse model by long-term high fat diet and explore the characteristics of glucose and lipid metabolism.Methods The mice were treated with high fat diet for three months.Their serum levels of sugar and lipid,and index of liver and fat to body weight were also measured.The quantitive analysis of mRNA expression of genes related to lipid metabolism was performed by real-time PCR.Results Compared with control group serum glucose level,serum lipid level and index of fat to body weight were remarkably increased in the group of high fat diet,while the expression of CPT1 mRNA in liver and musle were reduced（P 0.05）.Conclusion Long-term high fat diet could induce hyperlipidemia and the abnormality of glucose tolerance in mice.