冠心合剂对人脐静脉内皮细胞分泌血管生长因子bFGF的影响

Impact of Guanxin Decoction on Secretion of Angiogenesis Factor(bFGF) of Human Umbilical Vein Endothelial Cells

目的:观察冠心合剂对人脐静脉内皮细胞(HUVEC)分泌血管新生因子bFGF的影响。方法:取SD大鼠40只,随机分为冠心合剂大、中、小剂量和空白组,每组10只,冠心合剂大、中、小剂量组以成人用药剂量的20、10、5倍剂量灌胃,共7天,末次灌胃后各组大鼠下腔静脉取血制备含药血清。采用体外培养HUVEC的方法,将HUVEC随机分为冠心合剂大、中、小剂量组、空白对照组和bFGF对照组,加入含药血清24h后,通过MTT法、ELISA法和RT-PCR法观察冠心合剂对HUVEC的增殖率、bFGF含量及bFGF mRNA表达的影响。结果:冠心合剂大剂量组HUVEC增殖率、bFGF含量及bFGFmRNA表达量与空白对照组比较,差异有统计学意义(P<0.05,P<0.01),但其作用不及bFGF对照组(P<0.05)。结论:冠心合剂能提高HUVEC增殖率,增加bFGF含量,促进bFGFmRNA表达,进而促进血管新生。

Objective：To observe the effect of Guanxin Decoction on human umbilical vein endothelial cells（HUVEC） to secret angiogenesis factor of basic fibroblast growth factor（bFGF）.Methods：Medicated sera were collected from Sprague Dawley rats intragastrically administered 20（high dose,group A）,10（medium dose,group B）,and 5（low dose,group C） times of adult dosage of Guanxin Decoction,respectively.HUVEC were incubated in vitro,and then they were stimulated by group A,B,and C medicated sera.HUVEC without stimulation and bFGF positive HUVEC served as control groups.After HUVEC were stimulated by medicated serum for 24 h,the MTT method,enzyme-linked immunosorbent assay method（except the bFGF positive control group） and RT-PCR method were adopted to observe the impacts of different doses of Guanxin Decoction on proliferation of HUVEC,secretion of bFGF,and expression of bFGF mRNA.Results：In group A and B,the proliferation of HUVEC was markedly promoted as compared with normal control group（P0.05,P0.01）,but the proliferation was no better that of the bFGF positive control group（P0.05）.The secretion of bFGF and the expression of bFGF mRNA in group A were significantly higher than that of the normal control group（P0.05）.Conclusion：Guanxin Decoction may promote angiogenesis by elevating the proliferation of HUVEC and the content of bFGF.