摘要
目的:通过比较准分子激光角膜表层切削术(Epi-LASIK)及PRK术后角膜上皮及基质的愈合过程,探讨Epi-LASIK术后上皮瓣对角膜创后修复的影响。方法:中华大耳白兔33只,随机分为观察组30只,正常对照组3只。观察组兔随机一眼行Epi-LASIK,另一眼行PRK,术后1、7天,1、3、6个月观察上皮下雾状混浊(Haze)情况;于各时相点处死动物取角膜并行HE染色,通过角膜组织病理学观察比较角膜上皮及基质愈合情况。正常对照兔不作手术。结果:术后Epi-LASIK组上皮愈合过程较PRK组缓慢(P<0.05),Epi-LASIK组Haze程度较PRK组轻;角膜组织病理学显示,Epi-LASIK组术后3～6个月时角膜上皮厚度及角膜前基质细胞数明显低于PRK组(P<0.05)。结论:Epi-LASIK上皮瓣在术后角膜创面的愈合中有重要作用。
Objective：To investigate the effect of corneal flap on corneal wound healing after laser-assisted surface keratectomy.Methods：A total of 30 Chinese rabbits（66 eyes） were randomly received Epi-Lasik in one eye and PRK in the other eye.Other 3 rabbits（6 eyes） served as normal controls.Haze was evaluated at different postoperative time points（1 d,7 d,1 month,3 months,and 6 months）.Six rabbits were sacrificed at each time point and the healing process of corneal epithelium and stoma were evaluated by HE staining.Results：The epithelial healing process of the Epi-Lasik eyes was slower than that of the PRK eyes,while the Haze level of the Epi-lasik eyes was lighter than that of the PRK eyes.Pathological analysis showed that the corneal epithelial thickness and number of stromal cells of the Epi-Lasik eyes in 3 to 6 months after operation were significantly lower than those of the PRK eyes（P0.05）.Conclusion：The corneal flap plays an important role in the corneal wound healing progress after Epi-Lasik.
出处
《浙江中西医结合杂志》
2011年第9期613-615,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省宁波市医疗卫生科技专项(No.2008C50011)
