PLGA-磷酸钙盐复合材料的细胞相容性研究

Cell Compatibility of Composite Biomaterials Composed of PLGA and Calcium Phosphate Compound

用小鼠E13.5磨牙牙胚细胞作为种子细胞,观察4种生物材料对牙胚细胞的细胞黏附情况及其对牙胚细胞增殖分化的影响.体外实验表明PLGA/HA有利于细胞黏附,体内实验表明PLGA/HA有利于细胞增殖,PLGA/TCP和PLGA/CDHA有利于细胞分化.最终认为PLGA/TCP和PLGA/CDHA的细胞相容性最好,可望成为牙齿再生的生物材料.

The mouse E13.5 molar tooth germ cells were accessed as the seeding cells. The attachment efficiency, distribution of cells after attaching, the effect of different biomaterials on the proliferation and differentiation of tooth germ cells were evaluated. The in vitro assay demonstrated chat PLGA/HA was more useful for cell attachment, the in vivo assay demonstrated that PLGA/HA was more useful for cell proliferation, and PLGA/TCP and PLGA/CDHA were useful for cell CDHA have good cell compatibility differentiation. In conclusion, and it＇s hopeful to be used in PLGA/TCP and PLGA/ the tooth regeneration