CARD11与Bcl-2在弥漫性大B细胞淋巴瘤中的表达与意义

Study on the expression and signicance of CARD11 and Bcl-2 in diffuse large B-cell lymphoma

目的观察弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma)中CARD11与Bcl-2的表达情况,探讨它们在弥漫性大B细胞淋巴瘤中不同免疫分型中的表达及相互关系。方法选取弥漫性大B细胞淋巴瘤60例和反应性增生的淋巴结20例,采用组织芯片技术和免疫组织化学方法对弥漫性大B细胞淋巴瘤进行免疫分型并检测弥漫性大B细胞淋巴瘤免疫学类型GCB和ABC型中CARD11和Bcl-2表达情况。结果 (1)免疫分型60例弥漫性大B细胞淋巴瘤中免疫类型GCB25例,ABC35例。(2)60例弥漫性大B细胞淋巴瘤中免疫类型中CARD11与Bcl-2的表达情况:免疫类型GCB中CARD11的阳性率为24.0%,ABC中CARD11的阳性率为57.4%,两者之间P<0.05;GCB中Bcl-2的阳性率为32.0%,ABC中Bcl-2的阳性率为62.9%,两者之间P<0.05。结论①CARD11、NF-κB高表达与弥漫性大B细胞淋巴瘤的ABC类型相关。

Objective To study the expression and signicance of CARD11 and Bcl-2 in the common immunology types of diffuse large B-cell lymphoma and to explore the corrlativity during them.Methods 60 specimens of our diffuse large B-cell lymphoma patients and 20 specimens of reactive hyperplasia lymphoid node were selected,CARD11 and Bcl-2 were studied by using tissue chip technique and the immunohistochemical methods.Results（1）In 60 specimens of diffuse large B-cell lymphoma patients.immunology type：GCB 25 cases,ABC35 cases.（2）GCB 25 cases,the positive rates of CARD11 was 24.0%,ABC 35 cases,the positive rates of CARD11 was57.4%,there was significant difference between the two groups（P〈0.05）;GCB 25 cases,the positive rates of Bcl-2 was 32.0%,ABC35 cases,the positive rates of Bcl-2 was 62.9%,there was significant difference between the two groups（P〈0.05）.Conclusion The high expression of CARD11 and Bcl-2 were related to ABC immunology type of diffuse large B-cell lymphoma.