摘要
考察了亲水性离子液体/Tris-HCl缓冲液混合溶剂体系中简单节杆菌催化甾类化合物17α-羟基-16β-甲基孕甾-4,9(11)二烯-3,20-二酮(简称HMPDD)的C1,2脱氢生成17α-羟基-16β-甲基孕甾-1,4,9(11)三烯-3,20-二酮(简称HMPTD)的反应过程,通过细胞相容性和对反应影响的研究,选择了[EMIM](L)-Lac作为用于该体系的离子液体,并对离子液体体积分数、初始底物质量浓度、菌体质量浓度和反应时间等条件进行了优化.结果表明,在含体积分数为0.3%的[EMIM](L)-Lac的Tris-HCl缓冲液(0.05mol/L,pH 7.5)体系中,当菌体干重质量浓度为13.1g/L,底物质量浓度为4g/L时,简单节杆菌催化HMPDD脱氢反应16h的转化率可达89.9%,较Tris-HCl缓冲液介质中的脱氢转化率提高10%,且反应时间缩短了8h.
The dehydrogenation of 17α-Hydroxy-16β-methylpregna-4, 9 （ 11 ）-diene-3, 20-dione （HMPDD） to 17α-Hydroxy- 16β-methylpregn-1,4,9 （11）-triene-3,20-dione （HMPTD） catalyzed by Arthrobacter simplex was performed in a mixed solvent containing ionic liquid and Tris-HC1 buffer（0.05 mol/L, pH 7.5）. The influences of some reaction parameters were investigated. The optimal values of [EMIM] （L）-Lac content, cell concentration, substrate concentration and reaction time are 0.3%, 13.1 g/L, 4 g/L and 16 h, respectively. Under the optimum conditions, a maximum conversion rate of 89.9% was obtained,with an increase of 10% compared to that in Tris-HC1 buffer. The reaction time with maximum conversion rate was 8 h shorter in the ionic liquids containing system.
出处
《浙江工业大学学报》
CAS
北大核心
2011年第5期532-535,540,共5页
Journal of Zhejiang University of Technology
基金
浙江省自然科学基金资助项目(Y407289)
