β-arrestin 2对急性支气管哮喘小鼠模型脾CD4^+T细胞T-bet表达的影响

Effect of β-arrestin 2 on T-bet expression of splenic CD4^+T cells in asthmatic murine model

目的探讨β-arrestin 2在急性支气管哮喘(简称哮喘)小鼠模型脾CD4+T细胞中的表达及对T-bet表达的影响。方法建立急性哮喘小鼠模型,免疫磁珠分离小鼠脾源性CD4+T细胞,RNA干扰β-arrestin 2的表达后,检测CD4+T细胞β-arrestin 2、T-bet mRNA和蛋白的表达。结果 siRNA-β-arrestin 2-1123沉默效果最佳,相对于未转染siRNA的哮喘组及其他siRNAβ--arrestin 2转染组,β-arrestin 2 mRNA明显降低(P<0.01);哮喘小鼠模型脾CD4+T细胞β-arrestin 2蛋白的表达明显高于正常组(P<0.01);β-arrestin 2沉默后,哮喘小鼠脾CD4+T细胞T-bet mRNA和蛋白的表达明显升高(P<0.05)。结论β-arrestin 2可能通过抑制小鼠CD4+T细胞T-bet的表达参与哮喘的发病。

Objective To detect the expression of β-arrestin 2 in CD4＋T cells in acute asthmatic murine models as well as the effect of β-arrestin 2 on expression of T-bet.Methods Splenic CD4＋T cells origining from asthmatic murine models were isolated by immunomagnetic bead separation.After β-arrestin 2 expression was knocked down by RNA interference,β-arrestin 2 as well as T-bet mRNA and protein expression were determined.Results siRNA-Arrb2-1123 had better silencing effect than other siRNA sequences.β-arrestin 2 expression in splenic CD4＋T cells in asthmatic murine models was significantly higher than that of the normal group（P0.01）.After β-arrestin 2 expression was knocked down by RNA interference,T-bet mRNA and protein expression in splenic CD4＋T cells in asthmatic mice were significantly increased（P0.05）.Conclusions β-arrestin 2 probably contributes to the pathogenesis of asthma by inhibiting T-bet expression in CD4＋T cells.