基于拟杆菌特异性16S rRNA基因的塘坝型饮用水污染溯源研究

Potential Use of Bacteroidales Specific 16S rRNA in Tracking the Rural Pond-drinking Water Pollution

微生物溯源是通过比较污染样品与可能的污染源中粪便污染指示微生物的差异或其生物标记的有无来判断污染样品和可能污染源之间存在的联系,从而确定污染来源。鉴于传统的溯源方法操作复杂、耗时长,建立了一种基于拟杆菌群体特异性16S rRNA基因进行溯源的方法,利用该方法证明了水源周围的池塘对饮用水的污染贡献较大。与已报道的另一种新的快速溯源方法——利用大肠杆菌特异性基因phoE(膜外周磷通道蛋白编码基因)的PCR-DGGE技术进行比较研究的结果表明,利用拟杆菌特异性16S rRNA基因的PCR-DGGE溯源方法结果可靠、操作简便,较之大肠杆菌phoE基因的PCR-DGGE溯源方法,拟杆菌的溯源方法更适合塘坝型饮用水的溯源研究。

Microbial source tracking（MST） is based on the differences of indicator bacteria or special band from environment samples and suggested production source. It provide accesss to track the sources of fecal pollution. Traditional MST methods is complex and time-consuming. In order to explore a quick an easy method, we practised to analyse the differences in Bacteroidales communities based on specific gene 16S rRNA by PCR-DGGE. The results indicated the drink water pollution were contributed by the pond. Additionally, this method was compared with reported PCR-DGGE which based on Escherichia coli specific gene phoE （the outer membrane phosphoporin protein coding gene）. The results showed that acteroidales specific gene 16S rRNA could get more accurate result in investigated samples, which hinted that the researched method was more reliable and practical to tracking pollution.