沂州木瓜初代培养技术研究

Studies on the Initial Culture Technology of Yizhou Pawpaw

利用沂州木瓜新长出10d左右的嫩茎为外植体,采用不同消毒剂、灭菌时间、不同浓度6-BA对其进行初代培养试验。结果表明:沂州木瓜最佳消毒药剂及时间是70%酒精10s+0.1%升汞2～4min。当NAA浓度为0.05mg/L时,沂州木瓜试管苗生长适宜的6-BA浓度为0.5mg/L。

The 10 days new buds of Yizhou pawpaw were used as the explants.These explants were cultured primarily with different disinfectors and different sterilizing time and different concentration of 6-BA.The results showed that it was the best for the explants to treat with 70% alcohol for 10 s and 0.1% HgCl2 for 2~4 min.When the concentration of NAA was 0.05 mg/L,the suitable concentration of 6-BA for Yizhou pawpaw test-tube plantlet growth was 0.5 mg/L.