摘要
以木霉菌出发菌株T21及作用方式不同木霉菌REMI突变株为试材,研究了对番茄灰霉病菌作用方式不同木霉菌突变株间孢子萌发和产孢能力的差异以及对不同温度、pH值的适应性和对碳氮源的利用能力。结果表明:突变株Ttrm68孢子萌发率最高,为98%;突变株Ttrm25产孢能力最强,极显著高于出发菌株T21。不同木霉菌突变株对温度适应性有所改变,菌株Ttrm68表现出耐高温,在温度高达40℃时仍能生长。不同木霉菌突变株在不同pH值下的培养性状和产孢情况有所改变,菌株Ttrm68在碱性条件下能产厚垣孢子。不同木霉菌突变株对不同碳氮源的利用能力改变较大,菌株Ttrm68能够充分利用各种碳氮源,生长速度最快。
Original strain T21 and the different mode of action REMI mutants of Trichoderma were used as test material,biological characteristics of different Trichoderma strains including sporulation and its conidia germination,and the effect of different temperature,pH and nitrogen and carbon materials on Trichoderma＇s growth were studied.The results showed that germination ratio of Ttrm68 was the highest,reached to 98%.The conidia yield of Ttrm25 was the highest,which was significantly higher than that of wild strain T21.There were some change on Trichoderma strains growth under different temperature and Ttrm68 showed the high-temperature resistance and could grow at 40℃.The culture and sporulation of different Trichoderma strains had some change under different pH and Ttrm68 and T21 could produce chlamydospore.There were great change on the utilization of different Trichoderma strains to different carbon and nitrogen materials.Ttrm68 could utilize all kinds of carbon and nitrogen materials and had a fast growth.
出处
《北方园艺》
CAS
北大核心
2011年第17期150-153,共4页
Northern Horticulture
基金
辽宁省博士科研启动资助项目(20051051)
