摘要
利用RAPD分子标记技术对9个黄伞菌株进行遗传多样性分析。结果表明:RAPD技术是准确评估黄伞遗传多样性的有效方法。50条RAPD引物中筛选出8条多态性引物,共检测出226条带,其中多态性条带143条,多态率为63%。菌株之间遗传相似系数(GS)变幅范围为0.6181～0.9306,平均GS值为0.746,表明黄伞遗传变异较丰富。聚类分析结果表明,在相似系数0.763处可将9个黄伞菌株划分为4类。
Random amplified polymorphic DNA（RAPD） molecular markers were used to detect the genetic diversity among nine Pholiota adiposa strains.The results showed that eight primers worked well on stability and polymorphism were filtered for RAPD,the total number of band was 226,among which the number of the polymorphic band was 143,occupied with 63%.The genetic similarity among the strains ranged from 0.6181~0.9306,with a mean of 0.746,indicating very high genetic diversity in Pholiota adipose.The cluster analysis results showed that nine Pholiota adipose strains were classified into four groups with GS 0.763.RAPD technique might be an effective method to evaluate the genetic diversity of Pholiota adipose.
出处
《北方园艺》
CAS
北大核心
2011年第17期187-189,共3页
Northern Horticulture
基金
天津市农业科学院院长基金资助项目(06008)
