摘要
[目的]研究茉莉酸诱导的钙动员通往IP3敏感的钙离子通透性通道的作用。[方法]以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中,利用LASAF(Leica Application Suite-Advanced Fluorescence)软件记录肝素对茉莉酸(JA)诱导的胞内钙离子荧光强度的变化。[结果]经不同浓度的肝素预处理后,拟南芥叶细胞中胞内钙离子的荧光强度降低,再用100μmol/LJA处理时,其荧光强度升高,但仅与未经肝素处理的荧光强度相当。[结论]肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。
[Objective] This study was to investigate the role of IP3 sensitive calcium channel in the JA-induced calcium mobilization pathway.[Method] Arabidopsis thaliana leaves were labeled by Fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2+ which was pretreated with heparin on jasmonic acid(JA)-induced.[Results] When A.thaliana leaf cells were pretreated with 10,50 or 100 ng/ml heparin,intercellular free Ca2+ fluorescence intensity was reduced in comparison with negative control.Once the heparin-pretreated A.thaliana leaf cells were stimulated with 100 μmol/L JA,intercellular Ca2+ fluorescence intensity increased gradually and tended to be stable at a degree equivalent with that in negative control.[Conclusion] The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2+ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.
基金
Supported by National Natural Science Foundation of China(30700428,30911130166)
Natural Science Foundation of Beijing Municipality(5072009)
The New Star Plan of Science and Technology in Beijing Municipality(2006B26)~~
关键词
拟南芥
钙离子
肝素
茉莉酸
Arabidopsis thaliana
Ca2+
Heparin
Jasmonic acid