表没食子儿茶素没食子酸酯对哮喘小鼠呼吸道变应性炎症作用的研究

Effects of epigallocatechin-3-gallate on the allergic airway inflammations in mice models

目的探讨表没食子儿茶素没食子酸酯(EGCG)对尘螨卵清蛋白(OVA)致敏/激发小鼠呼吸道变应性炎症的作用。方法 C57BL/6小鼠以OVA致敏/激发,在其每日供水时加入EGCG;在最后一次激发24 h后,留取标本,检测支气管肺泡灌洗液(BALF)中的细胞计数和分类,测定BALF、血浆中细胞因子水平以及外周血和BALF中Foxp3+CD4+T细胞的比例。结果哮喘小鼠可见明显炎性细胞浸润,经EGCG治疗后肺部炎性细胞浸润显著减少;BALF中炎性细胞,特别是嗜酸性粒细胞计数较对照组明显升高(P<0.01);白细胞介素(IL)-4、IL-5水平较对照组明显升高(P<0.01),Foxp3+T细胞比例显著下降(P<0.01),Th1细胞因子干扰素-γ(IFNγ-)无显著变化,治疗组与模型组相比细胞计数、IL-4和IL-5水平、Foxp3+T细胞比例显著增加,与对照组相比无显著差异;IL-10水平显著升高。结论 EGCG可有效抑制哮喘模型小鼠呼吸道变应性炎症,其作用途径可能是通过诱导Foxp3+T细胞的产生和IL-10的分泌,进而抑制Th2型免疫反应,这提示EGCG也许可以应用于哮喘的辅助治疗。

Objective To investigate the effects of epigallocatechin-3-gallate （EGCG） on C57BL/6 mice models with airway al lergic inflammation sensitized and challenged with ovalunfin （OVA）. Methods C57BL/6 mice were sensitized and challenged with OVA and treated with EGCG. 24 hours after the last time of sensitization, samples of bronehoalveolar lavage fluid （BAI.F） were collected and detected for cell counts and classification. Levels of eytokines in BAI.F and plasma and the ratio of Foxp3^＋ CD4^＋ T cells in peripheral blood and BALF were detected. Results There was obvious infiltration of inflammatory cells in model mice group and the infiltration declined after be treated with EGCG. Compared with control group,total cells counts,eosinophil counts and levels of Il- 4 and IL-5 in BALF of model group enhanced significantly,levels of IL-10 decreased significantly,while levels of IFN-7 unchanged. In EGCG treated group,the infiltration of inflammatory cells and levels of IL-4 and IL-5 decreased, cytokines of Thl un- changed and the percentages of Foxp3 ^＋ T cells increased significantly. Conclusion EGCG could efficiently inhibit the allergic air- way inflammations in model mice,which might be induced by the inhibition of type Th2 immunereaction through the emerging of Foxp3^＋ T ceils and the secretion of IL-10. EGCG might be able to utilized for the adjunctive therapy of asthma.