T淋巴细胞亚群绝对值测定CD45/SSC设门法和CD3/SSC设门法的比较研究

Comparsion of CD45/SSC and CD3/SSC gating methods on absolute count of T-lymphocyte subsets

目的比较流式细胞仪两种设门方法测定外周血中T淋巴细胞亚群绝对值的差异。方法收集20例患者的外周全血,分别采用CD45/SSC、CD3/SSC两种设门方法测定T淋巴细胞亚群的百分率和绝对值,将同一标本测得的两个结果进行配对t检验,并对T淋巴细胞亚群绝对值做进一步分析,计算其线性方程。结果两种方法测得的CD3+、CD3+CD4+淋巴的绝对值差异无统计学意义(P>0.05);但CD3+CD8+淋巴的绝对值差异有统计学意义(P<0.05)。两种方法测定CD3+、CD3+CD4+、CD3+CD8+淋巴绝对值的相关性良好。结论两种方法测定CD3+、CD3+CD4+淋巴的绝对值结果一致,均可作为CD3、CD4细胞绝对值的测定方法,并且可以相互补充。

Objective To compare two different gating method on the absolute count of T lymphocyte subsets in peripheral blood. Methods Peripheral blood collected from 20 cases of patients were detected by using flow cytometry （F（FCM） with CD45/ SSC and CD3/SSC gating method respectively for percentage and absolute count of T-lymphocyte subsets. Two results,detected by two gating methods,of the same sample, were statistically analyzed by paired t test. The coefficients of correlation for absolute T cells were calculated. Results There were no significant differences between the absolute counts of CD3 ^＋ and CD3 ^＋ CD4^＋ cells de tected by the two methods （P〉0.05）. But there was difference of the absolute counts of CD3^＋ CD8^＋ cells （P〈0.05）. Conclusion There was fine coincidence between the two gating methods for the absolute counts of CD3^＋ and CD3^＋ CD4 ^＋ cells. These two ga ring methods could both used and complement for each other for the absolute counts of CD3 and CD4 cells.