人乳头瘤病毒基因亚型在宫颈病变患者中的检测及分析

Detection and analysis of human papillomavirus genetype distribution in patients with cervical disease

目的了解宫颈病变患者人乳头瘤病毒（HPV）感染状况及其亚型分布，为宫颈癌的防治提供理论依据。方法采用核酸分子快速导流杂交基因芯片技术（HybriMax）对622例宫颈病变患者宫颈脱落细胞进行21种HPV亚型的检测。结果宫颈病变患者中HPV感染306例，感染率49．2％，高危型HPV感染278例，感染率44．7％；各高危亚型在HPV阳性患者中的栓出率由高到低排序为：HPVl6、58、18、52、31、68、59、39、56型，其他各高危亚型未检出。宫颈病变I～Ⅱ期与Ⅲ～Ⅳ期之间及病变细胞分化程度之间，HPV阳性率差异无统计学意义（P〉0．05）。结论宫颈疾病患者中有相当的比例感染HPV，由于高危亚型与宫颈病变关系密切，加强HPV检查对有效降低宫颈癌的发生率具有重要意义。

Objective To investigate the state of human papillomavirus （HPV） infection and distribution of HPV genotype in patients with cervical disease. Methods Flow-through hybridization and gene chip technology were used to detect the 21 genotypes of HPV in cervical cells from 622 cases of patients with cervical disease. Results 306（49.2%） cases were HPV positive in 622 patients with cervical disease. 278（44.7%） cases were associated with high risk types of HPV. The order of frequency of high risk types of HPV in patients with cervical disease was HPV16,58,18,52,31,68,59,39 and 56, other subtypes were not found. HPV positive rates were not statistically different between clinical stages I -II and Ⅲ--IV ,or between differentiations degree of cells （P〉0.05）. Conclusion HPV positive rate was very high in patients with cervical disease. The screening of HPV infection should be strengthened in order to reduce the incidence of cervical cancer and mortality as the closer relationship between cervical disease and infection of high risk type HPV.