摘要
目的评价ELISA法、快速血浆反应素试验(RPR)、梅毒螺旋体明胶颗粒凝集试验(TPPA)3种方法在梅毒检测中的应用价值。方法应用ELISA、RPR和TPPA 3种方法对175例梅毒患者和120例非梅毒患者进行检测,比较3种检测方法的灵敏度、特异度、假阴性率和假阳性率。结果 RPR法、ELISA法及TPPA法的灵敏度分别为74.28%、100.00%和100.00%;特异度分别为78.33%、96.66%和100.00%。ELISA法和TPPA法检测梅毒的灵敏度和特异度均高于RPR法,差异均有统计学意义(P<0.05)。RPR法的假阴性率和假阳性率分别为25.71%和21.66%,均高于ELISA法和TPPA法。结论 RPR法不适于梅毒抗体的筛查试验,ELISA法可替代RPR法作为梅毒检测的筛查试验,TPPA法可作为ELISA法阳性的确证试验。
Objective To evaluate the clinical value of ELISA,rapid plasma regain test(RPR) and treponema pallidum particle assay(TPPA) in the detection of syphilis. Methods The serums of 175 syphilitic cases and 120 non syphilis patients were detected by EI.ISA,RPR and TPPA. Then, the sensitivity, specificity, false positive rate and false negative rate of differenl melhods were compared. Results The sensitivity and specificity were 74.28% and 78.33% for RPR, 100.0% and 96.66% for ELISA, 100.0 and 100.0% for TPPA. The sensitivity and specificity of ELISA and TPPA were higher than RPR(P〈0.05). The false negative rate and the false positive rate of RPR were 25. 71% and 21. 66 %, which were higher than ELISA and TPPA. Conclusion RPR was not suitable for syphilis antibody screening. ELISA could be used for syphilis screening,and TPPA was suitable for confirming positive samples,detected by EI.ISA.
出处
《国际检验医学杂志》
CAS
2011年第15期1718-1719,共2页
International Journal of Laboratory Medicine