儿茶素提取新工艺

Study on the New Extraction Process of Catechin

优选用溶剂萃取法提取、测定茶叶中儿茶素的方法。以乙醇为浸提液,采用香草醛一浓盐酸法测定茶叶中儿茶素的含量,并通过正交实验确定提取茶叶中儿茶素的最佳工艺条件。儿茶素溶液的最大吸收波长为506 nm。儿茶素浓度与吸光度间的回归方程为:A=0.058X+0.059,R2=0.994。正交实验结果表明,儿茶素的最佳提取和纯化条件为:在pH值为4的条件下,以60%的乙醇为溶剂,料液比为1∶15(g/mL),于60℃下恒温浸提30 min。影响儿茶素含量的因素依次为:pH值>乙醇浓度>浸提时间>料液比>提取温度。通过研究茶叶中儿茶素的提取、测定方法,确定了经济、高效、实用的提取分离工艺,建立了简便、准确的儿茶素含量测定方法。

To study the optimum conditions for extracting catechin in tea by solvent extraction method.With ethanol as leaching liquor.The method of vanillin-hydrochloric acid was used to determine the content of catechin in tea and the optimum technique conditions for extracting catechin in tea was confirmed by orthogonal test.The maximum absorption wavelength of catechin solution Was 506 nm.The regression equation between catechin concentration and absorbance was： A=0.058X＋0.059,R2=0.994.The results of orthogonal test sho-wed that the optimum extracting and purifying conditions for catechin were as follows： extracting with 60 % ethyl alcohol as solvent at the pH of 4,extracting at the temperature of 60 ℃ for 30 min,the ratio of material to liquid of 1∶15（g/mL）.The factors that affected the content of catechin were as follows： pH valueethanol concentrationextraction timethe ratio of material to liquidextraction temperature.By studying the extraction and determination of catechin in tea.We determined a economic,efficient and practical extraction and separation process.And established a simple,accurate of catechin determination methods.