乙型肝炎病毒基因型与耐药病毒株的相关性研究

Study of the correlation between HBV genotypes and the resistance to lamivudine

目的研究乙型肝炎病毒(HBV)基因型与耐药病毒株产生的相关性。方法征集340例接受拉米夫定治疗1年以上的慢性乙型肝炎(CHB)患者为研究对象。采集血样经离心分离血浆,进行HBV DNA定量检测,再进行基因测序和基因分型。结果基因测序和基因型分析结果为:A型16例(4.7%),B型112例(32.9%),C型148例(43.5%),D型44例(12.9%),B/C混合型20例(5.9%)。其中发生YMDD耐药突变的基因型分别是:A型4例(25.0%),B型20例(18.5%),C型64例(42.1%),D型8例(18.2%),B/C混合型4例(20.0%)。结论 HBV基因型C发生YMDD耐药突变的频率较高,HBV基因型与YMDD突变可能存在相关性。

Objective To explore the correlation between hepatitis B virus （HBV） genotypes and the drug resistance. Methods 340 patients with chronic hepatitis B （CHB）, receiving LAM therapy for more than one year,were enrolled and detected for the plasma HBV DNA level,and HBV DNA were extracted and amplified for sequencing and genotyping. Results Sequencing and gen- otyping revealed that there was a different prevalent frequency of HBV infected genotypes. 16 （4.7 ~//00 ） cases were with genotype A, 112（32.9%） with genotype B,148（43.5~/~） with genotype C,44（12.9~//oo） with genotype D and 20（5.9%） with mixed genotype B/ C. The frequency of mutations in YMDD motif in patients with different genotypes were 4（25.0 %） in genotype A, 20（18.5 %） in genotype B, 64 （42.1 ~ ） in genotype C, 8 （18.2 ~ ） in genotype D and 4 （20.0 ~ ） in mixed genotype B/C. Conclusion The frequen- cy of mutations in YMDD motif in HBV genotypes C were higher than that in the others. There could be a correlation between HBV genotype and mutations in YMDD motif.