摘要
文章采用50%甲醇溶解,超声波超30 min,再用流动相为乙腈-0.4%磷酸溶液(80:20),色谱柱为Ultimate AQ-C18(4.6×250,5μm),检测波长为327 nm,流速1.0 ml/min,柱温25℃。在该色谱条件下,绿原酸在2.85～14.05(μg/ml)范围内线性良好,相关系数为0.9998,加样回收率为90.6%,RSD1.02%,该方法简单,易操作,分离度好,适用于金银花中绿原酸的测定。
This paper uses 50% methanol solution, ultrasonic over 30min, and then mobile phase of acetonitrile -0.4% phosphoric acid （80：20）, column for the Ultimate AQ-C18 （4.6×250, 5 tμm）, detection wavelength of 327nm,flow rate 1.0ml/min, column temperature 25℃. In the chromatographic conditions of chlorogenic acid in the 2.85-14.05 （μg / ml ） a good linear range, correlation coefficient of 0.9998, recovery was 90.6%, RSD1.02%, the method is simple and easy to operate, separation Well,for chlorogenic acid determination.
出处
《企业技术开发》
2011年第3期6-7,共2页
Technological Development of Enterprise
关键词
高效液相
金银花
绿原酸
HPLC
Honeysuckle
Chlorogenic acid
