不同性激素状态致去势小鼠前列腺组织形态学改变

Histomorphological Change of Prostate Induced by Different Sex Hormone in Castrated Mice

目的观察不同性激素状态下去势小鼠前列腺组织形态学变化。方法将40只成年balb/c雄性小鼠随机分为正常对照组(N组),去势对照组(C组),去势雄激素组(T组),去势雌激素组(E组)和去势雌雄比例组(R组)。处理4周后,测量血中雌、雄激素含量;观察各组小鼠前列腺指数及前列腺组织学变化;结果和正常组比较,去势后给予不同性激素处理,前列腺指数均有所增加,其中以去势雌雄合剂组最为明显。病理结果显示去势雄激素组前列腺上皮增生明显,间质增生不明显;去势雌激素组前列腺间质肌层增生明显,和正常前列腺比较,上皮成份明显减少;去势雌雄合剂组腺上皮和间质均明显增生,腺体和血管周围可见炎症细胞浸润。结论不同性激素状态下前列腺增生的成份和程度有所不同。其中,雄激素使前列腺上皮增生明显,间质增生不明显;雌激素使前列腺间质肌层增生明显,上皮成份明显减少;雄激素和雌激素共同作用使腺上皮和间质均明显增生,并伴有炎症细胞浸润。

Objective This study aimed to observe the histomorphological change of prostate induced by different sex hormone in castrated mice.Methods A total of 40 balb/c mice were randomly divided into 5 groups： normal control（Group N）;castration group（Group C）;β-estradiol treated group（Group E）;testosterone treated group（Group T）;β-estradiol and testosterone treated group（Group R）.Four weeks later,serum androgen and estrogen levels were assayed by ELISA.The prostate indexes were calculated and histological change was examined by HE staining.Results In comparison with normal group,the prostate indexes were increased in groups treated with different sex hormone,which was most obvious in Group R（P0.05）.Epithelial cell atrophy was noted in Group C by HE staining.Remarkable prostatic epithelial hyperplasia was observed in Group T but stromal hyperplasia is not obvious.The marked stromal hyperplasia was seen in Group E and prostatic epithelium was significantly reduced relative to normal prostate.Both Prostatic epithelial and stromal hyperplasia were observed in Group R.In addition,inflammatory infiltration around the vessels and acini was viewed in Group R.Conclusion The components and degrees of prostatic hyperplasia were different with sex hormone status.The androgen mainly caused the prostatic epithelial hyperplasia but not the stromal hyperplasia and the obvious stromal muscle＇s hyperplasia around the anci was seen after the estrogen treatment.The epithelial and stromal hyperplasia associated with inflammatory infiltration were induced by the treatment with androgen and estrogen.