摘要
目的探讨并总结制作Langendorff离体心脏灌注模型的要点及成功经验。方法通过16只健康SD大鼠(体质量200～400 g),以HTK液作为心脏停搏液建立标准的Langendorff离体心脏灌注模型,将此模型与持续K-H液心肌灌注、持续心脏搏动组大鼠的同一时段的心肌酶学及血流动力学指标进行对比,使用SPSS 11.5作统计学重复测量资料的方差分析。结果 1例因操作不熟练致提取心脏时间延长,1例因灌注管插入深度过深导致主动脉瓣毁损,1例因室温过低,1例因灌注管管径过大无法插入而导致实验失败,其余12例在应用了室内加热器、不同管径灌注针、自制球囊测压管及加快操作速度后均获得实验成功。所制模型心肌酶学及血流动力学各指标与持续心脏搏动组无明显差异(P>0.05)。结论掌握制作要点及注意事项后,Langendorff离体心脏灌注模型可顺利快速地成功制备。
Objective To explore and summary successful points for establish Langendorff heart perfusion models.Methods Through 16 healthy SD rats（200-400g each）,using HTK solution for cardioplegia and establishing standard Langendorff heart perfusion models,and compare myocardial enzymes and hemodynamic with continuous perfusion and beating group.Results 1 case failed because of unskilled operation in extracting the heart;1 case failed because of inserting the perfusion-tube too deeply to damage the aortic valve;1 case failed because of lowly indoor-temperature;1 else failed because of excessive diameter of perfusion-tube;12 cases prepare successfully with the application of indoor heaters,perfusion needles with different diameters,self-made balloons and accelerating the operations.There＇s no significant difference between cardiac resuscitation group and continuous heart beat group in myocardial enzymes and hemodynamic.Conclusion After mastering the attentions above,isolated heart perfusion models can be established successfully and quickly.
出处
《实验动物科学》
2011年第4期60-62,65,共4页
Laboratory Animal Science
基金
云南省科学技术厅联合专项课题:硝酸甘油与H0E-642和Verapamil配伍对供心保存效果的实验研究(No:2009CD179)
关键词
离体心脏
灌注模型
制备
isolated heart
perfusion model
establish
