抗幽门螺杆菌UreB蛋白免疫乳的制备

Application of anti-Helicobacter pylori UreB subunit vaccine to prepare functional immune milk

诱导UreB蛋白克隆菌株表达UreB蛋白,并制备成亚单位疫苗,免疫正常泌乳奶牛,测定乳清中特异性抗体效价以及牛乳常规成分、血清常规成分等健康指标.结果表明:UreB蛋白表达浓度为0.39mg/mL;乳清特异性抗体IgG和IgM效价显著高于对照组(P<0.05),IgA效价有所上升,但未达到显著水平(P>0.05);奶牛血清中各项生理生化指标没有显著变化(P>0.05),乳清中除非脂型固形物差异显著外(P<0.05),其余指标没有显著差异(P>0.05).说明幽门螺杆菌UreB亚单位疫苗可以有效提高牛奶中特异性抗体的浓度,并对奶牛健康及牛奶成分没有显著影响.

The UreB protein was expressed by UreB colonies,and was used to prepare UreB subunit vaccine.The normal lactating dairy cows were immunized with UreB subunit vaccine.Determination of specific antibody titer whey,the milk composition and serum composition such as health indicators were measured.The results showed that UreB protein was expressed and purified successfully with the concentration of 0.39 mg/mL.Serum specific IgG and IgM titers of milk were significantly higher（P0.05） than those of control groups,specific IgA antibody increased compared to the control group（P0.05）.The results of blood and milk composition of dairy cows showed that no difference was observed between the cow serum indexes and the control group（P0.05）,except that Non-fat solids was lower（P0.05）,other indicators was not significantly affected（P0.05）.The Helicobacter pylori UreB subunit vaccine could effectively improve the milk protein-specific antibodies,and no significant effects werer observed on cow health and milk composition.