人参皂苷对人肺腺癌细胞转化生长因子β1表达的影响

The role of Giusenoside Rg3 on apoptosis and the levels of transformin growth factor-β1 in A549 cell line

目的探讨人参皂苷对人肺腺癌A549细胞及其对转化生长因子β1（TGF-β1）表达的影响。方法将人肺腺癌标准细胞株A549培养后完全随机分为人参皂苷组、对照组，采用四氮唑蓝法检测各组细胞增殖率；用酶联免疫吸附法测定各组细胞TGF—β1的表达水平，并分析TGF-β1的表达与A549细胞凋亡的相关性。结果当人参皂苷浓度为0．1、1、10、100μmoL／L时，A549细胞内TGF—β1表达水平逐渐降低，其吸光度（A）值分别为2．712±0．285、1．436±0．285、0．543±0．280、0．125±0．022，不同浓度的人参皂苷组间比较及其与对照组（A值为3．737±0．234）比较，差异均有统计学意义（均P〈0．05）。当人参皂苷浓度为1、10、100μmol／L时，A549细胞半数抑制浓度（IC50）分别为0．26±0．09、0．54±0．04和0．74±0．10，与阴性对照组（0．01±0．01）相比，差异均有统计学意义（均P〈0．05）。结论人参皂苷可以抑制肿瘤细胞TGF-β1的分泌，从而诱导肿瘤细胞凋亡。

Objective To investigate the role of ginsenoside Rg3 inducing apotosis in lung A549 cell line and the expression of transformin growth factor （TGF）-β1 levels. Methods Rg3-induced group and the control group were included in this study. 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） was used to determine the effect of Rg3 on apoptosis. The expression of TGF-β1 was analyzed by Enzyme-linked Immunosorbent Assay （ELISA）. Results MTT assay showed that compared with the control group the apoptosis of Rg3-treated cells dramatically increased. Compared to the control group, the expression of TGF-β1 in the Rg3-treated decreased obviously. Conclusion Ginsenoside Rg3 has a high inhibition in the expression of TGF-β1 in A549 cell line and can induce the apotosis.