聚乙二醇干扰素治疗慢性乙型肝炎疗效与人类白细胞抗原DRB1等位基因的相关性研究

The relationship between efficacy of PEG-interferon and HLA-DRB1 alleles in the patients with chronic hepatitis B

目的探讨青岛地区汉族慢性乙型病毒性肝炎患者聚乙二醇干扰素治疗疗效与人类白细胞抗原（HLA）-DRB1等位基因的关系。方法以接受聚乙二醇干扰素α-2a正规治疗48周的青岛地区汉族慢性乙型肝炎患者40例作为研究对象，应用聚合酶链反应-序列特异性引物技术对患者的HLA-I／类抗原的DRB1 ＊04、＊07进行等位基因检测，应用SC2000统计软件对结果进行分析。结果40例慢性乙型肝炎患者接受聚乙二醇干扰素α-2a治疗48周后，完全应答者19例（47．5％），部分应答者10例（25．0％），无应答者11例（27．5％）。无应答者11例中，HLA—DRB1＊04携带者4例，HLA-DRB1＊07携带者7例；部分、完全应答者29例中，HLA—DRB1＊04携带者1例；HLA—DRB1＊07携带者6例，无应答者HLA-DRB1＊04和＊07携带率均明显高于部分、完全应答者（前者P〈0．05，OR=16．0；后者P〈0．05，OR=6．7）。结论青岛地区汉族人群中慢性乙型肝炎聚乙二醇干扰素α-2a治疗无应答与HLA-DRB1＊04、＊07呈正相关。

Objective To study the relationship between efficacy of PEG-interferon α-2a for forty-eight weeks and some HLA alleles （ HLA-DRB1 ） in the patients with chronic viral hepatitis B from Qingdao of the Han nationality, and provide immuno-genetic knowledge for the prevention and cure of hepatitis B virus infection. Methods Forty patients with chronic viral hepatitis B in a Qingdao population of the Han nationality were subjected to a standard course of treatment with PEG-interferon a-2a for 48 weeks. HLA-DRB1 ＊ 04, ＊ 07 were detected hy using the polymerase chain reaction-sequence specific primer （PCR-SSP） technique. The data were analyzed by SC2000 software. Results After forty patients with chronic viral hepatitis B were subjected to a course of treatment with PEG-interferon α-2a for 48 weeks, there were nineteen patients in the complete-responders, ten patients in the partial-responders and eleven patients in non-responders. The patients of HLA-DRB1 ＊ 04 in eleven non-responders were four and in twenty-nine partial-or complete-responders was one, The frequency of HLA-DRB1 ＊ 04 in non-responders was higher than that in the partial-or complete-responders （P 〈 0.05, OR = 16.0）. The patients of HLA- DRB1 ＊ 07 in eleven non-respotlders were seven and in twenty-nine partial-or complete-responders were six. The frequency of HLA-DRB1 ＊ 07 in non-responders was higher than that in the partial-or complete-responders （P 〈 0.05, OR = 6.7 ）. Conclusions In a Qingdao population of the Han nationality, no response of PEG-interferon α-2a therapy in the patients with chronic viral hepatitis B was correlated positively with HLA-DRB1 ＊ 04, ＊ 07.