鸡源金黄色葡萄球菌噬菌体分型的研究

A STUDY ON PHAGE TYPING OF STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM CHICKENS

本试验应用噬菌体分型的方法,对主要来源于我国京津地区葡萄球菌病鸡的207株金黄色葡萄球菌进行了分型。 应用国际金黄色葡萄球菌噬菌体分型系统分型的结果为:常规检验稀释度(R.T.D.)时分型率71.12％,其中属噬菌体第Ⅰ裂解群的最多,Ⅲ群次之,混和群和Ⅳ群(又不定群)再次之,属Ⅱ群者最少(分别占可分型总株数的48.98％,18.24％,14.12％,12.84％和5.41％)。在可分型菌株中,不同分离部位(主要为皮肤病灶、内脏和眼)的菌株均以属噬菌体裂解群Ⅰ群的居多;来源自不同日龄(主要为雏鸡、育成鸡和成鸡)的菌株也以属噬菌体裂解群Ⅰ群者居多。同时发现,鸡源金黄色葡萄球菌菌株中仅能被52A型噬菌体强烈裂解的占可分型总数的44.22％。 试验说明,自我国京津地区分离的鸡源金黄色葡萄球菌菌株与人源株有一定差异,但具有较好的同源性,文中讨论了两者间交叉感染的可能性。

Phage typing was performed on 207 strains of Staphylococcus aureus isolated from chickens in Beijing and Tianjin areas, China, using the International S.aureus phage set.71.12% strains could be typed with the International S .aureus phage set ( at R.T.D. ) .of these typable strains, 48.98% belonged to phage lytic group Ⅰ, 18.24% to groupⅢ, 14.12% to mixed group, 12.84% to group Ⅳ and 5.41% to group Ⅱ. Of the strains isolated from the various lesions ( skin lesions, internal organs and ill eyes ) and differently aged chickens(totally 3 groups: 1-40 days, 41-120 days and over 120 days old ) , the largest amount of strains belonged to phage lytic group Ⅰ , respectively. It was discovered that, of the S .aureus strains isolated from chickens and could be typed with the international phage set,44.22% strains could be strongly lysed by phage 52A.The experiment revealed that there was some diference between S. aureus strains isolated from chickens in Beijing and Tianjin areas and those from human origin. But the strains, isolated from chickens and human beings, had some relationship. The possibility of crossinfection of Staphylococcosis between chickens and human beings was discussed.