鄂西北产苍耳子甲醇粗提物抑菌活性及其清除DPPH能力的初步评价

Primary Evaluation of Antifungal and DPPH-Eliminating Activity of Crude Methanol Extracts of Xanthium sibiricum Seeds from Northwestern Hubei

采用生长速率法、孢子萌发法及DPPH自由基清除法,对产自鄂西北的野生植物苍耳子粗提物体外抑菌活性及抗氧化性进行了初步测定。结果表明,苍耳子甲醇粗提物对绿色木霉、黄瓜灰霉菌、黑曲霉、终极腐霉、尖镰孢菌黄瓜专化型五种病原真菌均有一定的抑制作用,其中无论是抑制菌丝生长还是孢子萌发,均对黑曲霉显示出了显著的抑制活性。实验结果也表明,苍耳子甲醇粗提物对DPPH自由基具有较强的清除能力I,C50为每毫升0.000468 g。

The antifungal activities of crude methanol extracts of Xanthium sibiricum seeds from northwestern Hubei were primarily measured through mycelia growth method and spore germination method aganinst the pathogenic fungi Trichoderma viride,Botrytis cinerea,Aspergillus niger,Pythium ultimum and Fusarium oxysporum f.sp cucumber.The results showed that the crude methanol extracts of X.sibiricum seeds could function some inhibitory activities to all above mentioned fungi.Among which,the most significant was Aspergillus niger in the inhibitory activities of both mycelia growth and spore germination.However,the inhibitory activities against the mycelia growth ould spore germination of the five fungi increased with the higher extract concentration.And the extract antioxidant activity was evaluted as well by eliminating DPPH free radical.According to the testing,X.sibiricum seed methanol extracts had the stronger DPPH-eliminating activity and IC50 was 0.000468 g·ml-1.