期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

金龟子绿僵菌甘露糖6-磷酸异构酶基因cDNA序列的克隆及分析

Cloning and Sequence Analysis of Mannose-6-phosphate Isomerase cDNA from Metarhizium anisopliae ZJ1109
下载PDF
导出
摘要 通过绿僵菌属甘露糖6-磷酸异构酶基因保守核苷酸区域设计简并性引物,采用RT-PCR及RACE-PCR技术成功克隆了金龟子绿僵菌mpi基因cDNA序列。该基因cDNA序列全长为1 513 bp,开放阅读框长度为1 328 bp,共编码441氨基酸。BLAST分析发现该基因演绎的氨基酸序列与其它真菌同源性较高,蛋白结构分析表明MPI蛋白是较保守的蛋白磷酸酶结构特征,主要由α螺旋和不规则卷曲构成。 The primers were designed according to the conservative sequence of mannose-6-phosphate isomerase gene,and the complete cDNA sequence of Metarhizium anisopliae was amplified by RT-PCR and RACE PCR.The complete cDNA sequence of mpi gene was 1513 bp and the complete ORF length was 1328 bp which encoded a protein with 441 amino acid residues.Blast analysis indicated the deduced amino acid sequence of the mpi gene from M.anisopliae showed high homology with other fungi respectively.The analysis of protein structure showed MPI protein had the characteristic of the conservative phosphate enzyme,and mainly constructed by α-helix and random coil.
作者 李亚
机构地区 广东海洋大学农学院
出处 《广东海洋大学学报》 CAS 2011年第4期17-22,共6页 Journal of Guangdong Ocean University
基金 广东省自然科学基金(9452408801002439) 湛江市科技计划项目
关键词 金龟子绿僵菌 RACE 甘露糖6-磷酸异构酶基因 克隆 Metarhizium anisopliae RACE mannose-6-phosphate isomerase gene cloning
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献15

  • 1李增智,樊美珍.真菌生物技术与真菌杀虫剂的发展[M].北京:科学出版社,2000.
  • 2Clarkson J M, Chamley A K. New insights into the mechanisms of fungal pathogenesis in insects[J]. Trends Microbiology, 1996, 4(5) : 97-203.
  • 3Wang C, St. Leger R J. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses[J]. Proceedings of the National Academy of Sciences USA 2006, 103(17): 6647-6652.
  • 4Empadinhas N, Marugg J D, Borges N, et al. Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archeon Pyrococcushorikoshii biochemical and genetic characterization of keyenzymes[J]. The Journal of Biological Chemestry, 2001, 276(47): 43580-43588.
  • 5Gill J F, Deretic V, Chakrabarty A M. Overproduction and assay of Pseudomonas aeruginosa phosphomannose isomerase[J]. Journal of Bacteriology, 1986,167(2): 611-615.
  • 6Pitkanen J P, Torma A, Alff S, et al. Excess mannose limits the growth of phosphomannose isomerase PMI40 deletion strain of Saccharomyces cerevisiae[J]. The Journal of Biological Chemestry, 2004, 279(53): 55737-55743.
  • 7Fang W, Yu X, Wang B, et al. Characterization of the Aspergillus fumigatus phosphomannose isomerase Pmil and its impact on cell wall synthesis and morphogenesis[J]. Microbiology 2009, 155(10): 3281-3293.
  • 8毛新国,景蕊莲,孔秀英,赵光耀,贾继增.几种全长cDNA文库构建方法比较[J].遗传,2006,28(7):865-873. 被引量：47
  • 9Korbie D J, Mattick J S. Touchdown PCR for increased specificity and sensitivity in PCR amplification[J]. Nature Protocols, 2008, 3(9): 1452-1456.
  • 10Hecker K H, Roux K H. High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR[J]. Biotechniques, 1996, 20(3): 478-485.

二级参考文献29

  • 1毛新国,孔秀英,赵光耀,贾继增.利用改进的Cap-trapper法构建拟斯卑尔脱山羊草(Ae.speltoides Tausch)全长cDNA文库[J].Acta Genetica Sinica,2005,32(8):811-817. 被引量：10
  • 2Haas B J,Volfovsky N,Town C D,Troukhan M,Alexandrov N,Feldmann K A,Flavell R B,White O,Salzberg S L.Full-length messenger RNA sequences greatly improve genome annotation.Genome Biol,2002,3(6):RESEARCH0029.
  • 3Burge C B,Karlin S.Prediction of complete gene structure in human genomic DNA.J Mol Bio,1997,268:78～94.
  • 4Lukashin A V,Borodovsky M.GeneMark.hmm:new solutions for gene finding.Nucleic Acids Res,1998,26(4):1107～1115.
  • 5Salzberg S L,Pertea M,Delcher A L,Gardner M J,Tettelin H.Interpolated Markov models for eukaryotic gene finding.Genomics,1999,59(1):24～31.
  • 6Carninci P,Shibata Y,Hayatsu N,Sugahara Y,Shibata K,Itoh M,Konno H,Okazaki Y,Muramatsu M,Hayashizaki Y.Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes.Genome Res,2000,10(10):1617～1630.
  • 7Seki M,Carninci P,NishiyamaY,Hayashizaki Y and Shinozaki K.High-efficiency cloning Arabidopsis full-length cDNA by biotinylated CAP trapper.The plant Journal,1998,15(5):707～720.
  • 8Carninci P,Waki K,Shiraki T,Konno H,Shibata K,Itoh M,Aizawa K,Arakawa T,Ishii Y,Sasaki D,Bono H,Kondo S,Sugahara Y,Saito R,Osato N,Fukuda S,Sato K,Watahiki A,Hirozane-Kishikawa T,Nakamura M,Shibata Y,Yasunishi A,Kikuchi N,Yoshiki A,Kusakabe M,Gustincich S,Beisel K,Pavan W,Aidinis V,Nakagawara A,Held WA,Iwata H,Kono T,Nakauchi H,Lyons P,Wells C,Hume D A,Fagiolini M,Hensch TK,Brinkmeier M,Camper S,Hirota J,Mombaerts P,Muramatsu M,Okazaki Y,Kawai J,Hayashizaki Y.Targeting a complex transcriptome:the construction of the mouse full-length cDNA encyclopedia.Genome Res,2003,13 (6B):1273～1289.
  • 9Stapleton M,Liao G,Brokstein P,Hong L,Carninci P,Shiraki T,Hayashizaki Y,Champe M,Pacleb J,Wan K,Yu C,Carlson J,George R,Celniker S,Rubin G M.The Drosophila gene collection:Identification of putative full-length cDNAs for 70% of D.melanogaster genes.Genome Res,2002,12(8):1294～1300.
  • 10Kikuchi S,Satoh K,Nagata T,Kawagashira N,Doi K,Kishimoto N,Yazaki J,Ishikawa M,Yamada H,Ooka H,Hotta I,Kojima K,Namiki T,Ohneda E,Yahagi W,Suzuki K,Li C J,Ohtsuki K,Shishiki T,Otomo Y,Murakami K,lida Y,Sugano S,Fujimura T,Suzuki Y,Tsunoda Y,Kurosaki T,Kodama T,Masuda H,Kobayashi M,Xie Q,Lu M,Narikawa R,Sugiyama A,Mizuno K,Yokomizo S,Niikura J,Ikeda R,Ishibiki J,Kawamata M,Yoshimura A,Miura J,Kusumegi T,Oka M,Ryu R,Ueda M,Matsubara K,Kawai J,Carninci P,Adachi J,Aizawa K,Arakawa T,Fukuda S,Hara A,Hashizume W,Hayatsu N,Imotani K,Ishii Y,Itoh M,Kagawa I,Kondo S,Konno H,Miyazaki A,Osato N,Ota Y,Saito R,Sasaki D,Sato K,Shibata K,Shinagawa A,Shiraki T,Yoshino M,Hayashizaki Y,Yasunishi A.Collection,mapping,and annotation of over 28 000 cDNA clones from japonica rice.Science,2003,301(5631):376～379.

共引文献50

广东海洋大学学报
2011年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部