响应面优化黑葵花籽壳中黄酮的提取及抗氧化性研究

Optimization of extraction conditions of flavonoids from black sun-flower seed shell by response surface method and its antioxidant properties

利用响应面法对黑葵花籽壳中黄酮类化合物的提取条件进行了优化,得出了黑葵花籽壳黄酮类化合物的最佳工艺条件。结果表明:从黑葵花籽壳中提取黄酮类化合物的最佳乙醇浓度为70%vol,最佳提取温度为50℃,提取时间为2h,料液比为1∶23(v/w)。在此条件下提取率由5.6%提高到6.2%。对葵花籽壳黄酮的抗氧化研究表明:葵花籽壳黄酮的还原能力和超氧阴离子自由基(O.)的清除作用均明显高于2,6-二叔丁基对甲酚(BHT);在质量浓度小于8.0mg/L时,葵花籽壳黄酮对羟基自由基(.OH)的清除率高于BHT,而在质量浓度大于8.0m/L时低于BHT。

Extraction conditions of flavonoids from black sun-flower seed shell were optimized by response surface methodology. The result showed that the optimal extraction conditions were as follows： alcohol concentration 70%vol, extraction temperature 50℃, extraction time 2h and solid liquid ratio l：23（v/w） and the extraction rate increased from 5.6% to 6.2% under the conditions. Research on antioxidant properties showed that flavonoids of black sun-flower seed shell had better reducing ability and superoxide anion free radicals （O·） scavenging ability than that of butylated hydroxytoluene （BHT）. The flavonoids of black sun-flower seed shell had better ability of scavenging hydroxyl free radicals （· OH） than that of BHT when mass concentration less than 8.0mg/L, while it had lower effect than BHT when mass concentration was higher than 8.0mg/L.