金黄乳膏抗炎镇痛作用的实验研究

Experimental study on the anti-inflammation and analgesia effects of JINHUANG cream

目的:评价金黄乳膏的抗炎镇痛作用,探讨其作为金黄油膏取代产品的可行性。方法:①直接给药的抗炎作用。取小鼠28只,随机分为模型组、扶他林乳膏组、金黄油膏组、金黄乳膏组,每组7只。先用二甲苯致小鼠耳廓肿胀,再将相应药物敷于各组小鼠致炎侧耳部,随后处死小鼠计算耳肿胀度。②间接给药的抗炎作用。取小鼠24只,随机分成模型组、扶他林乳膏组、金黄乳膏组,每组8只。先将相应药物敷于各组小鼠腹部并固定,1.5 h后用二甲苯致小鼠耳廓肿胀,随后处死小鼠,测量并计算耳肿胀度。③不同载药量金黄乳膏的抗炎作用。取小鼠37只,随机分成模型组(n=7)、扶他林乳膏组(n=7)、金黄乳膏组(n=7)、金黄乳膏2.5倍量组(n=8)、金黄乳膏5倍量组(n=8)。先用二甲苯致小鼠耳廓肿胀,再将相应药物敷于各组小鼠致炎侧耳部,随后处死小鼠,测量并计算耳肿胀度。④镇痛作用。取小鼠37只,随机分为模型组(n=10)、扶他林乳膏组(n=9)、金黄油膏组(n=9)、金黄乳膏组(n=9)。先将相应药物敷于各组小鼠腹部并固定,连续给药3 d。第3天给药后2.5 h取下药膏,按0.01 mL.g-1体质量腹腔注射0.9%冰醋酸。记录注射后15 m in内小鼠的扭体次数。结果:①直接给药后,扶他林乳膏组、金黄油膏组和金黄乳膏组的耳肿胀度均小于模型组(P=0.000,P=0.002,P=0.000),其余各组间比较,差异均无统计学意义;②间接给药后,金黄乳膏组和扶他林乳膏组耳肿胀度均小于模型组(P=0.004,P=0.003),金黄乳膏组与扶他林乳膏组比较,差异无统计学意义(P=0.057);③测定不同载药量金黄乳膏的抗炎作用时,扶他林乳膏组、金黄乳膏组、金黄乳膏2.5倍量组和金黄乳膏5倍量组的肿胀度均小于模型组(P=0.000,P=0.000,P=0.000,P=0.000),其余各组间比较,差异均无统计学意义;④测定金黄乳膏的镇痛作用时,扶他林乳膏组、金黄油膏组和金黄乳膏组的扭体次数均少于模型组(P=0.002,P=0.000,P=0.000),其余各组间比较,差异均无统计学意义。结论:金黄乳膏保留了金黄油膏的基本疗效,对局部和全身都有良好的抗炎镇痛效果。从药效学角度来看,金黄乳膏作为金黄油膏的取代产品是可行的。

Objective：To evaluate the anti-inflammation and analgesia effects of JINHUANG cream,and to explore the feasibility of JINHUANG cream as the replacement product of JINHUANG ointment.Methods：①Anti-inflammation effects under direct administration：twenty eight mice were randomly divided into model group,voltaren cream group,JINHUANG ointment group and JINHUANG cream group,7 cases in each group.Ear swelling of mice were induced by xylene at first,and the inflammation parts of the ears were administrated with the relevant drugs,then the mice were sacrificed for the measurement of the ear swelling degrees.②Anti-inflammation effects under indirect administration：twenty four mice were randomly divided into model group,voltaren cream group and JINHUANG cream group,8 cases in each group.The relevant drugs were applied to and fixed on the abdomens of mice firstly,and 1.5 hours later,ear swelling of mice were induced by xylene,then the mice were sacrificed for the measurement of the ear swelling degrees.③Anti-inflammation effects of different dosage of JINHUANG cream：thirty seven mice were randomly divided into model group（n=7）,voltaren cream group（n=7）,JINHUANG cream group（n=7）,2.5 times dosage of JINHUANG cream group（n=8）and 5 times dosage of JINHUANG cream group（n=8）.Ear swelling of mice were induced by xylene at first,and the inflammation parts of the ears were administrated with the relevant drugs,then the mice were sacrificed for the measurement of the ear swelling degrees.④Analgesia effects：thirty seven mice were randomly divided into model group（n=10）,voltaren cream group（n=9）,JINHUANG ointment group（n=9）and JINHUANG cream group（n=9）.At first,the relevant drugs were applied to and fixed on the abdomens of mice for 3 days continuously,and the plaster were taken down from the mice 2.5 hours after the administration in the 3rd day,then the mice were intraperitoneally injected with 0.9%glacial acetic acid（0.01 mL·g-1）.The writhing times of the mice in the first 15 minutes after the injection were recorded.Results：①After direct administration,ear swelling degrees of mice in voltaren cream group,JINHUANG ointment group and JINHUANG cream group were all lower than those of mice in model group（P=0.000,P=0.002,P=0.000）,and there was no statistical difference between any other couple of groups.②After indirect administration,ear swelling degrees of mice in JINHUANG cream group and voltaren cream group were lower than those of mice in model group respectively（P=0.004,P=0.003）,and there was no statistical difference between JINHUANG cream group and voltaren cream group（P=0.057）.③The measurement result of the anti-inflammation effects of different dosage of JINHUANG cream showed that the ear swelling degrees of mice in voltaren cream group,JINHUANG cream group,2.5 and 5 times dosage of JINHUANG cream group were all lower than those of mice in model group（P=0.000,P=0.000,P=0.000,P=0.000）,and there was no statistical difference between any other couple of groups.④The measurement result of the analgesia effects of JINHUANG cream showed that the writhing times of mice in voltaren cream group,JINHUANG ointment group and JINHUANG cream group were all less than those of mice in model group（P=0.002,P=0.000,P=0.000）,and there was no statistical difference between any other couple of groups.Conclusion：JINHUANG cream retains the basic curative effects of JINHUANG ointment,and it has the satisfactory anti-inflammation and analgesia effects in the part and all over the body.From the pharmacodynamic point of view,it is feasible of JINHUANG cream to replace the JINHUANG ointment.