磷酸二氢根离子对香豆肼化合物的高选择性荧光增强

Fluorescent Emission of Coumaric Aldehyde Molecule Enhanced by Dihydrogen Phosphate

设计合成了荧光探针N,N-二乙基氨基香豆素-3-肼基-2,4-二硝基苯肼(DC)。光谱滴定实验显示,H2PO 4-与DC能够以1∶1的化学计量比形成配合物。进一步的实验发现,和相同含量的DC溶液相比,该配合物溶液的相对光致荧光强度显著增强,同时伴有紫外吸收光谱红移,溶液颜色由橙黄色迅速变为紫色。其它阴离子,如F-、NO3-、Cl-、Br-、I-等对DC溶液的相对荧光强度及紫外吸收光谱没有影响。利用DC对H2PO 4-具有灵敏选择性的特征,可把N,N-二乙基氨基香豆素-3-肼基-2,4-二硝基苯肼作为荧光探针,借助于荧光光谱和紫外-可见吸收光谱两种手段,实现对H2PO4-离子的快速识别。

By combining 7-diethylamino-2-oxo-2H-chromene-3-carbohydrazide with 2,4-dinitrophenylhydrazine into one molecule,fluorescent probe [7-diethylamino-2-oxo-N′-2,4-dinitrophenylhydrazine-2H-chromene-3-carbohydrazide]（DC） was synthesized for dsetection of H2PO-4 in acetonitrile.Spectrophotometric titration indicated the formation of a 1∶1 complex of H2PO-4 with DC.With the fluorescence spectra,the apparent red shift of UV absorption and the changes for the solution color from orange yellow to purple were observed as binding DC with H2PO-4.The experiment showed that DC was highly selective for H2PO-4,much more than those anions such as F-,NO-3,Cl-,Br-and I-.By the exclusive selectivity of DC to H2PO-4,it was predicted that as a fluorescent probe,and by means of fluorescence and UV-Visible absorption spectrum,H2PO-4can be rapidly identified by a enhanced fluorescence from DC.