泰山灰树花菌丝体多糖的微波辅助提取

MICROWAVE-ASSISTED EXTRACTION TECHNOLOGY OF POLYSACCHARIDES FROM GRIFLOLA FRONDOSA

利用微波辅助提取灰树花菌丝体多糖。以粗多糖的得率为考察指标,以时间、微波功率、液料比为考察因素,通过单因素实验和正交实验,确定微波辅助提取灰树花菌丝体多糖的最佳实验条件;采用硫酸-苯酚法对多糖含量进行了测定。微波辅助提取灰树花多糖正交实验优化结果为:时间6 m in,功率:200 W,料液比150:1,多糖得率为13.1%。微波辅助提取灰树花多糖与传统水提取法相比可缩短时间,提高粗糖产率,是一种有前景的多糖辅助提取方法。

To extract the mycelium polysaccharides from Griflola frondosa by the method assisted with microwave.In order to determine the optimal extraction condition,the conditions of polysaccharides extraction including extraction time,microwave power and ratw of water to material were studied by single factor experiment and orthogonal design;The polysaccharide was determined by phenol-sulphoacid method.The experimental results showed that the optimum extraction condition of mycelium polysaccharides from Griflola frondosa was as following： the extraction time is 6min,200W the miwowave power is the ratio of water to material is,150：1 the polysaccharides extraction rate was 13.1%.Compared with the traditional water extraction,microwave-assisted extraction of polysaccharide could reduce the time and increase the yield of raw polysacharide,and it was a promising assisted extraction method of polysaccharides.