白蜡绵粉蚧血细胞的显微形态与超微结构观察

The Microscopic Morphology and Ultrastructure of Hemocytes in the Mealybug Phenacoccus fraxinus

为研究蚧虫血淋巴中血细胞的种类及其结构特征,本文采用荧光显微镜、扫描电镜和透射电镜观察了白蜡绵粉蚧Phenacoccus fraxinus Tang(半翅目:蚧总科:粉蚧科)雌成虫血细胞的显微形态超微结构。结果显示,在荧光显微镜和扫描电镜下观察识别出白蜡绵粉蚧血淋巴中的5种血细胞,即原血胞、浆血胞、粒血胞、类绛色血胞和囊血胞。在透射电镜下,原血胞的细胞核明显,表现出高的细胞质密度;浆血胞最典型的特征是细胞质中有大量的囊泡;粒血胞的细胞质中有发达的粗面内质网和许多玫瑰形的细颗粒;类绛色血胞最典型的特征是有许多结晶,并在细胞质空泡区内分布更多;囊血胞透明性强,具有围核空间,膨大成潴泡状。

To understand the types and structures of blood cells in the haemolymph of scale insects,the morphology and ultrastructure of hemocytes collected from the adult mealybug,Phenacoccus fraxinus Tang（Hemiptera：Coccoidea：Pseudococcidae） were observed by fluorescence microscope,scanning electron microscopy and transmission electron microscopy.Five kinds of hemocytes,prophemocytes,graulocytes,plasmatocytes,cystocytes and oenocytoid,were observed in the haemolymph of the scale insect.Their typical characteristics were as follows：prophemocyte was the smallest one in the five types of the blood cells and it was with a clear cell nucleus and a high dense cytoplasmic;plasmatocyte was featured with a large number of vesicles in the cytoplasm;the cytoplasm of graulocytes had developed rough endoplasmic reticulum and many rose-shaped fine particles;oenocytoid was characterized by many crystalline in the cell;cystocytes had a circumference of nuclear space and swelling into a bubble retention.