大鼠肝再生相关基因MafF与EGFP融合表达研究

EGFP Fusion Expression of Rat Liver Regeneration Related Gene MafF

以大鼠再生肝为材料成功克隆到肝再生相关基因MafF的开放阅读框(ORF)。该ORF全长471bp,编码由157个氨基酸组成的蛋白质。基因芯片研究表明,MafF基因在部分肝切除后表达水平迅速升高。为了研究该基因在肝再生进程中的作用,构建其真核表达载体pEGFP-N1-MafF,在肝再生模型中进行融合表达。结果表明,转入后6h即可检测到融合蛋白,24h到达高峰,绘制了融合蛋白的表达动态,为进一步功能研究奠定基础。

A v-maf musculoaponeurotic fibrosarcoma oncogene homolog family member MafF was cloned by using RT-PCR method.The full length of ORF was 471 bp,encoding a protein consist 157 amino acid.To study its role during the rat liver regeneration,we construct an express vector,pEGFP-N1-MafF,and transferred into rat model of liver regeneration.Then sampling and making froze histological section at 6 h,12 h,24 h,and 48 h after transfer differently,calculate the transfer rate and observe the dynamic expression by fluorescent microscope.The fusion protein can be detected 6 h later,and peaked at 24 h after injection.