中药复方药物血清对CDKN2A和RPS3a基因在人卵巢癌细胞株SKOV3中表达的影响

Impacts of Chinese Herbal Compound Drug Serum on the Expressions of CDKN2A and RPS3a in Human Ovarian Cancer Cell Lines(SKOV3)

目的研究CDKN2A(细胞周期依赖性激酶抑制基因)和RPS3a基因(核糖体蛋白质S3a)在中药复方理冲生髓饮诱导人卵巢癌细胞株人卵巢浆液性乳头状囊腺癌(SKOV3)细胞凋亡中的作用。方法将20只Wistar大鼠随机分为理冲生髓饮组和生理盐水组,灌胃给药,采血制备含药血清。提取生理盐水组及理冲生髓饮组肿瘤细胞mRNA,制备DNA探针并分别用Cy3和Cy5两种荧光染料标记,与人细胞凋亡寡核苷酸微阵列芯片进行杂交,经扫描、分析,得出药物作用后表达有差异的基因。结果共筛选出显著差异表达基因66个,其中20个基因表达水平上调,46个基因表达水平下调。结论理冲生髓饮可能通过上调CDKN2A基因和下调RPS3a基因,达到诱导卵巢癌细胞凋亡的目的。诱导细胞周期停滞,阻碍肿瘤细胞蛋白质合成,影响细胞正常代谢等,可能是其诱导细胞凋亡的作用机制。

Objective To study the effects of CDKN2A（cyclin-dependent kinase inhibitor gene）and RPS3a（ribosomal protein S3a）on apoptosis in human ovarian serious cyctadenocarcinoma（SKOV3）induced by Chinese herbal compound formula,lichong shengsui yin.Methods 20 Wistar rats were randomized into a lichong shengsui yin group and a physiological saline group.The medication was done with gastric infusion.Blood was collected to prepare medicine-contained serum.mRNA of cancer cells in physiological saline group and lichong shengsui yin group were extracted.DNA probe pin was prepared and two fluorescent dyes,Cy3 and Cy5 were used for labeling separately.The hybridization was done with human apoptosis oligonucleotide microarray chip.Through scanning and analyzing,the genes with expression differences after medicinal action were obtained.Results 66 genes with significant differences were screened totally.Of them,the expressions of 20 genes were up-regulated and those of 46 genes were down-regulated.Conclusion Lichong shengsui yin works on apoptosis in ovarian carcinoma probably through the up-regulation of CDKN2A and down-regulation of RPS3a.It induces cyclin arrest,impedes cancer cell protein synthesis,affects normal metabolism of cells,etc.All of those may be involved in the mechanism of induced apoptosis.