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氧化葡萄糖酸杆菌实时定量PCR检测方法的建立与应用

Establishment and Application of Real-time PCR Assay for Quantification of Gluconobacter oxydans
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摘要 选取氧化葡萄糖酸杆菌16S rRNA作为看家基因,利用Primer Premier 5 0软件设计特异性引物,以总基因组为模板,建立氧化葡萄糖酸杆菌实时定量PCR方法。选取山梨醇脱氢酶大亚基.sldA和小亚基sldB为研究对象,以看家基因16S rRNA的表达作为内参,测得山梨醇脱氢酶大小亚基的转录水平一致,均为看家基因的68%。 A method using 16S rRNA as the housekeeping gene was established for the detection of Gluconobacter oxydans based on real-time quantitative PCR. Primers were designed by software Primer Premier 5.0 that are specific for the recognition of a conservative region in target gene. Sorbitol dehydrogenase containing two subunits, sldA and sldB, was detected and quantified with the transcriptional level of 16S rRNA. The result showed that the transcriptional level of these subunits were 68 % of that of the housekeeping gene.
作者 刘旭 魏东芝 林金萍
机构地区 华东理工大学生物反应器工程国家重点实验室
出处 《中国医药工业杂志》 CAS CSCD 北大核心 2011年第9期651-654,共4页 Chinese Journal of Pharmaceuticals
关键词 氧化葡萄糖酸杆菌 荧光定量PCR 山梨醇脱氢酶 Gluconobacter oxydans fluorescence quantitative PCR sorbitol dehydrogenase
分类号 Q783 [生物学—分子生物学]
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参考文献10

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中国医药工业杂志
2011年 第9期

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