摘要
目的探讨Bcl-2反义寡核苷酸能否增强K562细胞对依托泊苷的化疗敏感性。方法采用人工方法合成Bcl-2寡核苷酸,通过脂质体将Bcl-2不同寡核苷酸导入K562细胞中(正义寡核苷酸组、无义寡核苷酸组、反义寡核苷酸组),另设未经转染空白对照组,检测Bcl-2蛋白的表达和细胞凋亡率。采用不同浓度的依托泊苷作用于4组细胞,用MTT法测定细胞存活分数。结果空白对照组和正义、无义寡核苷酸组的K562细胞Bcl-2蛋白与Bcl-2蛋白条带相符,反义寡核苷酸组在相应位置未见蛋白条带,且凋亡率显著高于其他3组(P<0.01)。细胞与依托泊苷作用后,反义寡核苷酸组细胞在各个浓度的存活分数均低于其他3组(P<0.05)。结论 Bcl-2反义寡核苷酸能有效封闭Bcl-2基因表达,增加K562细胞的凋亡,增强依托泊苷的敏感性。
Objective To study the effect of Bcl-2 antisense oligonucleotide on chemotherapy sensitivity of Etoposide in cell line K562.Methods Different oligonucleotides were introduced into cultured K562 cells： antisense oligonucleotide group（ASODN group）,sense oligonucleotide group（SODN group）,non-sense oligonucleotide（NSODN group）,and blank control group without trasfection was established.The Bcl-2 protein and apoptosis rate were detected.After the 4 groups were treated by different concentrations of Etoposide,cell survival rate was detected by MTT.Results The expression of Bcl-2 protein blank control group,SODN group and NSODN group showed the same strap as Bcl-2 protein,but it was not found in ASODN group.Apoptosis rate in ASODN group was higher than that in the other 3 groups（P〈0.01）.After being treated with different concentrations of Etoposide,cell survival rate in NSODN group was lower than that in the other 3 groups（P〈0.05）.Conclusion The Bcl-2 antisense oligonucleotide can close the expression of Bcl-2 gene,increase the apoptosis of K562 cell line and enhance the sensitivity of etoposide.
出处
《临床误诊误治》
2011年第9期4-6,F0003,共4页
Clinical Misdiagnosis & Mistherapy
