摘要
采用非损伤微测技术,研究了沙冬青悬浮细胞经不同浓度的氯化钠、甘露醇处理后,细胞Ca2+、K+、H+3种离子流速、流向及流量的变化。结果表明:氯化钠与甘露醇处理均诱导Ca2+外流,氯化钠诱导的Ca2+外流与氯化钠浓度有关;氯化钠诱导K+外流,而甘露醇诱导K+内流;氯化钠诱导的H+流具有先内流后外流的两个阶段的特点。H+-ATP酶在氯化钠诱导的H+外流中具有重要作用。
Ion fluxes of Ca2＋ , K＋ and H＋ were studeied in Ammopiptanthus rnongolicus suspension cells in response to the treatments of NaCl and mannitol with different concentrations under laboratory conditions by using non-invasive micro-test technique. The results showed that Ca2＋ flux was efflux in response to the different NaCl and mannitol treatments. K＋ flux showed different patterns bettwen NaCl and mannitol treatments, NaCl induced K＋ efflux, however mannitol induced K＋ efflux. Biphasic H＋ flux was found under NaCl treatment, namely H＋ flux was subjected to the changes from efflux to influx. Inhibitor experiment showed that the ATP dependent H＋- pump played a significant role in H＋ efflux in response to salt stress.
出处
《西北林学院学报》
CSCD
北大核心
2011年第6期35-39,共5页
Journal of Northwest Forestry University
基金
国家自然科学基金(30871727)
关键词
沙冬青
非损伤微测技术
离子流
Ammopiptanthus mongolicus
non-invasive micro-test technique
ion flux
