新生儿溶血疾病的临床诊断方法的探讨

目的探讨新生儿溶血筛查试验在诊断新生儿溶血病(HDN)中的临床诊断方法,以明确其对本病早期诊断及临床症状程度判断的临床意义。方法采用微柱凝胶试验技术对疑为新生儿溶血病的125例黄疸患儿做直接抗人球蛋白试验、游离试验、放散试验。结果在125例患者中证实由血型抗原不合引起的新生儿溶血病患者45例(36%),其中ABO-HDN41例(91.1%),Rh-HDN4例(8.9%)。在41例ABO-HDN的患儿中,A型20例,占48.8%,B型21例,占51.2%。结论微柱凝胶检测技术具有操作简便、敏感性高、准确等优点,值得推广。

Objective To explore the clinical application of microcolumn gel technique in the diagnosis of neonatal hemolytic disease（HDN）detectingthe importance of the way of clinic diagnose.Methods Direct antiglobulin test,antibody release test and free antibody test were performed in 125cases suspected of HDN by using microcolumn gelassay.Results In 125 case,45cases of HDN（36%）caused by maternal-fetal blood type incompatibility were identified,in which 41 cases belonged to ABO-HDN（91.1%）and 4 case to Rh-HDN（8.9%）.In those ABO-HDN cases,20 cases（48.8%）were A blood type and 21 cases（51.2%）were B blood type.Conclusion The microcolumn Gel immunoassay is sensitive,simple and accurate.