期刊文献+

哺乳动物诱导多功能干细胞(iPSc)的技术研究进展 被引量:2

Research Technology Progress in Mammal Inducement of Pluripotent Stem CelIs
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摘要 诱导型多能干细胞(iPSc)具备胚胎干细胞的分化潜能,同时又回避了伦理问题,因此具有广泛且重要的临床应用价值。然而,目前iPS技术仍然存在致癌性、效率低等一系列问题。针对这些亟待解决的问题,本文综述了最近几年国内外在iPS细胞研究领域中的发展现状及研究方法,并探讨该领域中急需解决的问题和发展前景,以为诱导性多功能干细胞的研究提供一定的借鉴。 Induced pluripotent stem cells(iPSCs),having differentiation potential of the embryonic stem cell while avoiding the ethical issues,has wide and important clinical applications.However,there remain many problems to be solved for iPSCs technology itself,such as carcinogenicity,low efficiency of generation and so on.In order to solve the existent problems,the development and research methods in the international field of iPSCs,and the urgent problems and development prospects were reviewed here,so as to provide references for the future researches in this aspect.
出处 《中外医疗》 2011年第26期54-56,共3页 China & Foreign Medical Treatment
关键词 重编程 诱导多功能干细胞 研究进展 Reprogramming Induced pluripotent stem cells Advance
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参考文献17

  • 1Takahashi K.Induction of pluripotent stem cells from mouse em- bryonic and adult fibroblast cultures by defined factors[J].Cell, 2006,126(4):663- 676.
  • 2Kehat I.Human embryonic stem cells can differentiate into myocytes with structural and function properities of cardiomyocytes[J].Clinlnvest,2001,108:407 - 414.
  • 3Stadfeld M.Defining molecular cornerstones during fibroblast to iPS cell rep rogramming in mouse[J].Cell Stem Cell,2008,2(3): 230- 240.
  • 4陈艳玫,姚錱.转录因子Sox2的研究进展[J].生命科学,2004,16(3):129-134. 被引量:17
  • 5Nakagawa M.Generation of induced pluripotent stem cells with- out Myc from mouse and human fibroblasts[J].Nat Biotechnol, 2008,26:101- 106.
  • 6Shi Y.Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds[J]. Cell Stern Cell, 2008,3:568-574.
  • 7Carcy B W.Reprogramming of murine and human somatic cells using a single polycistronic vector[J].ProcNatl Acad Sci U S A, 2009,106:157- 162.
  • 8Nakagawa M. Generation of induced pluripotent stem cells with- out Myc from mouse and human fibroblasts[J].Nat Biotechnol, 2008,26:101- 106.
  • 9Danwei Huangfu.Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2[J].Nature,2008.
  • 10Soldner F. Parkinsoni-s disease patient-derived induced pluripo- tent stem cells free of viral reprogramming factors[J].Cell,2009, 136:964- 977.

二级参考文献30

  • 1Ambrosetti D C, Scholer H R, Dailey L, et al. Modulation of the activity of multiple transcriptional activation domains by the DNA binding domains mediates the synergistic action of Sox2 and Oct-3 on thefibroblast growth factor-4 enhancer.J Biol Chem, 200
  • 2Dailey L, Yuan H B, Basilico C. Interaction between a novel F9-specific factor and octamer-binding proteins is required for cell-type-restricted activity of the fibroblast growth factor 4 enhancer. Mol Cell Biol, 1994, 14:7758~7769
  • 3Ambrosetti D C, Basilico C, Dailey L. Synergistic activation of the fibroblast growth factor 4 enhancer by Sox2 and Oct-3 depends on protein-protein interactions facilitated by a specific spatial arrangement of factor binding sites. Mol Cell Biol, 1997, 1
  • 4Nishimoto M, Fukushima A, Okuda A, et al. The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2. Mol Cell Biol, 1999, 19:5453~5465
  • 5Schilham M W, van Eijk M, van de Wetering M, et al. The murine Sox-4 protein is encoded on a single exon. NuclAcidsRes, 1993, 21:2009
  • 6Botquin V, Hess H, Fuhrmann G, et al. New POU dimer configuration mediates antagonistic control of an osteopontin preimplantation enhancer by Oct-4 and Sox-2. Genes Dev,1998, 12:2073-2090
  • 7Yuan H B, Corbi N, Basilico C, et al. Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3. Genes Dev, 1995, 9:2635-2645
  • 8Collignon J, Sockanathan S, Hacker A, et al. A comparison of the properties of Sox-3 with Sry and two related genes, Sox1 and Sox-2. Development, 1996, 122:509-520
  • 9Koopman P, Gubbay J, Vivian N, et al. Male development of chromosomally female mice transgenic for Sry. Nature, 1991,351:117-121
  • 10Scaffidi P, Bianchi M E. Spatially precise DNA bending is an essential activity of the Sox2 transcription factor. J Biol Chem, 2001, 276:47296-47302

共引文献16

同被引文献42

  • 1刘洪智,周路纲,贺兰英,王磊,孙煜杰.周围神经联合活化巨噬细胞移植修复小鼠脊髓损伤[J].中国矫形外科杂志,2007,15(13):1015-1018. 被引量:3
  • 2Zain A,Sobani,Syed A. Stem cells for spinal cord regeneration:Current status[J].Surg Neurol Int,2010.93-99.
  • 3Ronaghi M,Erceg S,Moreno MV. Challenges of stem cell therapy for spinal cord injury:human embryonic stem cells,endogenous neural stem cells,or induced pluripotent stem cells[J].Stem Cells,2009,(01):93-99.
  • 4Bottai D,Cigognini D,Madaschi L. Embryonic stem cells promote motor recovery and affect inflammatory cell infiltration in spinal cord injured mice[J].Experimental Neurology,2010,(02):452-463.
  • 5Moreno MV,Rodriguez Jimenez FJ,Garcia RM. Activated spinal cord ependymal stem cells rescue neurological function[J].Stem Cells,2009.733-743.
  • 6Tetzlaff W,Okon EB,Karimi AS. A systematic review of cellular transplantation therapies for spinal cord injury[J].Journal of Neurotrauma,2010.1-72.
  • 7Lindsay SL,Riddell JS,Barnett SC. Olfactory Mucosa For Transplant-Mediated Repair:A Complex Tissue for a Complex Injury[J].Glia,2010.125-134.
  • 8Lima C,Escada P,Pratas VJ. Olfactorymucosal autografts and rehabilitation for chronic traumatic spinal cord injury[J].Neurorehabil:Neural Repair,2010.10-22.
  • 9Chen SJ,Chang CM,Tsai SK. Functional improvement of focal cerebral ischemia injury by subdural transplantation of induced pluripotent stem cells with fibrin glue[J].Stem Cells and Development,2010,(11):1757-1767.
  • 10Bradl M,Lassmann H. Oligodendrocytes biology and pathology[J].Acta Neuropathologica,2010.37-53.

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