胆道闭锁母源性微嵌合体的荧光原位杂交分析

The analysis of maternal microchimerism in biliary atresia using fluorescent in situ hybridization

目的探讨胆道闭锁与母源性微嵌合体的相关性。方法随机选取2010年1月至2010年8月经手术和病理活检的12例胆道闭锁和8例婴儿肝炎综合征、2例胆总管扩张症的男性肝脏组织石蜡切片中，采用荧光原位杂交染色体计数探针，标记细胞中的X、Y染色体，计数细胞核中2条X染色体的母体细胞。结果在12例胆道闭锁肝脏组织切片中均可发现母体细胞，在10例对照组肝脏组织切片中也可发现母体细胞；在肝脏组织切片的30个可读视野中，母体细胞的数量分别是11±2．59和1．8±1．69（P〈0．01），差异有显著统计学意义。结论胆道闭锁等男性患儿肝脏组织中均可发现数量不等的母源性微嵌合体；肝脏组织中母源性微嵌合体的数量，胆道闭锁显著高于其他肝病患儿；母源性微嵌合体可能与胆道闭锁的发病机制相关。

Objective To investigate the correlation between biliary atresia（BA） and maternal microchimerism. Methods Using the chromosome enumeration probes of fluorescent in situ hybridization（FISH）, the analysis of X and Y chromosomes were performed on liver paraffin sections of 12 male BA cases and 10 male controls, which involved 8 neonatal hepatitis and 2 choledochus cyst cases. Counted the maternal cells with XX chromosomes in each liver section. Results Maternal cells with XX chromosomes were found in liver sections of all male BA cases, while not always in male control cases. The maternal cells in per 30 views appropriate of BA group and control group were 11 ± 2. 59 and 1.8 ± 1.69, respectively（P〈0. 01 ）, demonstrating a significant statistical difference. Conclusions Maternal microchimerism could be found in the liver tissues of both BA and other infant hepatopathy cases. The quantity of maternal cells in the male BA livers is significantly higher than that in other neonatal liver diseases. Consequently, maternal microchimerism is suggested to contribute to the path- ogenesis of BA.