电针对兔膝骨关节炎软骨细胞基质金属蛋白酶-13表达的影响

Effect of electroacupuncture on expression of matrix metalloproteinase-13 in cartilage cells of rabbit knee osteoarthritis model

目的:探讨电针对兔膝骨关节炎(KOA)模型软骨细胞基质金属蛋白酶-13(MMP-13)表达的影响。方法:30只新西兰大耳兔随机分为正常组、模型组及电针治疗组。正常组不造模,模型组和电针治疗组均采用左膝关节伸直位管型石膏固定法,建立KOA模型。喂养6周后,电针组电针治疗10d。各组均取股骨内侧髁软骨,观察软骨细胞及MMP-13的变化。结果:按Mankin评分比较软骨结构,各组间差异有显著性(P<0.05)。正常组MMP-13未检出,模型组MMP-13检出率较高,电针治疗组MMP-13检出率下降。MMP-13在KOA软骨细胞中的表达与正常组差异有显著性意义,电针治疗组与模型组差异有显著性意义(P<0.01)。结论:电针治疗促使兔骨关节炎模型软骨细胞重新排列,减少软骨细胞中MMP-13表达,对OA治疗有一定作用。

Objective：To study the effect of electroacupuncture（EA） on expression of matrix metalloproteinase-13（MMP-13） in cartilage cells of rabbit knee osteoarthritis（KOA） model.Method：Thirty New Zealand big ear rabbits were divided into normal,model and EA group randomly.The left knees of model group and EA group were pasitioned with straight bit in plaster casts to create osteoarthritis（OA）,the normal group did not.After 6 weeks,EA group was carried on EA treatment for 10d.Then cartilage cells and MMP-13 changes of femoral medial condyle cartilage were observed in every group.Result：According to Mankin＇s grading to compare cartilage structure of 3 groups,there were significant differences（P0.05）.In normal group MMP-13 had not picked out.In model group detection rate of MMP-13 was high.In EA group detection rate of MMP-13 dropped.There were statistical significant differences of cartilage cell＇s MMP-13 expressions between model group and normal group,between EA group and model group（P0.01）.Conclusion：EA can promote rabbit KOA model cartilage cells rearrangement,reduce the expression of cartilage cells＇ MMP-13,and has certain effect on OA treatment.