纳米羟基磷灰石／聚酰胺66／成骨细胞／血管内皮生长因子165组织工程骨修复骨缺损再血管化与成骨活性

Revascularization and osteogenesis during repair of bone defects with tissue-engineered bone of nano-hydroaoatite crystai/polyamide 66/rabbit osteoplast/vascular endothelial growth factor

目的探讨以转染血管内皮生长因子165（vascular endothelial growth factor, VEGF165）基因的成骨细胞为种子细胞复合纳米网孔羟基磷灰石／聚酰胺66（nano - hydrxyapatite crystal/polyamide 66，n—HA／PA66）支架，构建的n—HA／PA66／成骨细胞／VEGF165组织工程骨修复兔桡骨缺损早期快速再血管化和成骨的能力。方法选新西兰大白兔56只，采用随机数字表法分成A、B两组，制作双侧桡骨骨缺损模型。A1组：将n—HA／PA66复合材料植入A组左侧骨缺损；A2组：将n—HA／PA66／VEGF265复合材料植入A组右侧骨缺损；B1组：将n—HA／PA66／成骨细胞／VEGF165复合材料植入B组左侧骨缺损；B2组：将11-HA／PA66／成骨细胞复合材料植人B组右侧骨缺损。术后2，4，8，12周进行大体观察，数字X线摄片（DR）、组织学切片、血管计数、扫描电镜观察。结果B1组各个时相点各种观察指标均表明其成骨活性及再血管化明显优于其他各组，差异有统计学意义（P〈0．05）。A1、A2组成骨活性及再血管化远不如B1、B2组，A1、A2两组之间差异无统计学意义。结论n—HA／PA66／成骨细胞／VEGF165组织工程骨具有良好的诱导成骨作用，在早期骨愈合中能促进新生血管快速形成，加快骨缺损的愈合。

Objective To employ the vascular endothelial growth factor （VEGF165） gene trans-fected rabbbit osteoplast to combine with the porous nano-hydrxyapatite crystal （n-HA）/polyam ide 66 （/PA66） so as to evaluate the osteogenesis and rapid revascularization early after repair of the rabbit radius bone defects with the tissue-engineered bone （n-HA/PA66/osteoplast/VEGF165）. Methods The animal models of bilateral radius bone defects were created in 56 New Zealand white rabbits that were then randomly divided into Group A and Group B. In Group A, the animals were implanted with n-HA/PA66 on the left bone defects （ Group A1 ） and with n-HA/PA66/VEGF165 composite materials on the fight bone defects （Group A2 ）. In Group B, the animals were implanted with n-HA/PA66/osteoplast/ VEGF165 composite materials on the left side （Group B1 ） and with n-HA/PA66/osteoplast on the right side （Group B2 ）. Gross, digital radiography, histological sections, vessel count and scanning electron microscopy （SEM） were performed at 2, 4, 8 and 12 weeks after operation. Results The osteogenesis and revascularization in Group B1 was superior to that in the other groups at each time point, with statistical difference （P 〈0.05）. The revascularization and osteogenesis in Groups B1 and B2 was far better than that in Groups A1 and A2, with no statistical difference between Group A1 and Group A2. Condusions The new tissue-engineered bone （n-HA/PA66/osteoplast/VEGF165） has a perfect osteogenetic effect and can promote rapid revascularization and the bone healing in the early stage after repair of the bone defects.