二甲双胍对肾癌细胞凋亡的影响及机制

Effect of metformin on apoptosis of renal cell carcinoma cells in vitro and its mechanisms

目的探讨二甲双胍对肾癌细胞凋亡的影响及其机制。方法通过Annexin V-FITC/PI染色镜下观察及流式细胞仪检测观察二甲双胍对786-O细胞凋亡的影响,通过免疫印迹检测AMPK信号及Caspase 9表达探讨可能分子机制。结果二甲双胍能够诱导786-O在低浓度血清环境凋亡,激活Caspase 9,二甲双胍能够激活786-O细胞中AMPK信号,但与其凋亡诱导效应无关。结论二甲双胍能够诱导肾癌细胞在低浓度血清环境凋亡,具有潜在治疗肾癌作用。

Objective To evaluate the effect of metformin on the apoptosis of renal cell carcinoma（RCC） cells in vitro and its mechanisms.Methods Fluorescent microscopy and flow cytometry were used to examine the changes in the apoptosis of 786-O cells after metformin treatment.The possible signaling molecules involved in this process were analyzed by immunoblot analysis of AMP-activated protein kinase（AMPK） signaling and caspase 9.Results Metformin induced apoptosis and caspase 9 activation in 786-O cells in low-serum medium but not in normal-serum medium.Metformin also induced AMPK activation in 786-O cells,but this activation was not associated with the cell proliferation inhibition or apoptosis-inducing effect of metformin.Conclusion Metformin can induce apoptosis of RCC cells in vitro,suggesting its potential as a therapeutic agent for RCC.