大蒜新素、双黄连对小鼠MHV-3性暴发型肝炎模型的作用

Preventive and therapeutic effects of Shuanghuanglian and allitride against experimental murine hepatitis virus type 3 infection in vivo

目的:探讨大蒜新素、双黄连在整体水平抗鼠肝炎病毒3型(MHV-3)感染效应.方法:将小鼠分为:大蒜新素、双黄连治疗组大蒜新素、双黄连预防组,大蒜新素、双黄连预防+治疗组,感染模型对照组,每组18只,及正常对照组,6只.建立MHV-3诱导的小鼠暴发型病毒性肝炎模型,通过观察模型小鼠存活时间、血浆ALT水平、肝脏病理改变和肝组织MHV-3病毒滴度变化,综合评估两种中药制剂的预防和治疗效应.结果:各组存活时间,血浆ALT水平、肝脏病理改变和肝组织MHV-3病毒滴度差异明显.大蒜新素预防组、双黄连预防组较模型组的病毒滴度(PFU/mg)明显减少(4.20±0.60,3.63±0.15vs6.07±0.25,均P<0.05);预防+治疗组较治疗组的病毒滴度(PFU/mg)也明显减少(3.70±0.44vs4.53±0.55,P<0.05);预防+治疗组较治疗组的病毒滴度(PFU/mg)明显减少(2.67±0.59vs3.77±0.31,P<0.05),预防组与预防+治疗组之间比较无明显差异.结论:大蒜新素、双黄连预防效应优于其治疗作用,可能作为冠状病毒流行期间人群预防的候选药物.

AIM： To study the preventive and therapeutic effects of Shuanghuanglian and allitride against hepatitis virus strain 3 （MHV-3） infection in a murine model.METHODS： BALB/c mice were divided into eight groups： Shuanghuanglian pretreatment group, allitride pretreatment group, Shuang- huanglian treatment group, allitride treatment group, Shuanghuanglian pretreatment and treat- ment group, allitride pretreatment and treatment group, model control group, and normal control group. Mice were injected intraperitoneally with MHV-3 （100 PFU/mouse） to induce MHV-3 infection. The survival time of tested mice and plasma ALT levels were recorded. Viral titers in liver tissue specimens were measured by plaque formation assay, and pathological changes were assessed by the Rezkalla method.RESULTS： Both drugs showed inhibitory activities against MHV-3 infection by significantly decreasing the viral load within liver tissue. The anti-MHV-3 effect of Shuanghuanglian was more significant in vivo. Pretreatment with the two drugs could improve the outcome of mice with fulminant hepatitis （4.20 PFU/mg ± 0.60 PFU/mg, 3.63 PFU/mg ± 0.15 PFU/mg vs 6.07 PFU/mg ± 0.25 PFU/mg; 3.70 PFU/mg ± 0.44 PFU/mg vs 4.53 PFU/mg ± 0.55 PFU/mg; 2.67 PFU/mg ± 0.59 PFU/mg vs 3.77 PFU/mg ± 0.31 PFU/mg, all P 〈 0.05）. CONCLUSION： Shuanghuanglian and allitride might be candidate preventive agents for community control during the epidemic period of SARS.